CatLogenzyme kinetics catalog

Values CatLog read from this paper

Effects of changing glutamate 487 to lysine in rat and human liver mitochondrial aldehyde dehydrogenase. A model to study human (Oriental type) class 2 aldehyde dehydrogenase

PMID 7910607doi:10.1016/s0021-9258(17)36725-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (43 values) from this paper: liver mitochondrial aldehyde dehydrogenase (class 2) from Rattus norvegicus, Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.2.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
liver mitochondrial aldehyde dehydrogenase (class 2)Homo sapiensNAD+Table I assay: 50 mM sodium phosphate—0.028—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Homo sapiensNAD+Table II two-substrate analysis: 100 mM sodium phosphate3.180.01320pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Homo sapienspropionaldehydeTable I assay: 50 mM sodium phosphate3.426 × 10⁻⁴5.7 × 10³ calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Homo sapienspropionaldehydeTable II two-substrate analysis: 100 mM sodium phosphate—8.2 × 10⁻⁴—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapiensNAD+Table I assay: 50 mM sodium phosphate; apparent Km; Table I—7.4—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapiensNAD+Table II two-substrate analysis: 100 mM sodium phosphate0.250.540.045pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapiensNAD+Table II two-substrate analysis: 100 mM sodium phosphate0.222.360.092pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapienspropionaldehydeTable I assay: 50 mM sodium phosphate; apparent Km; Table I0.320.0014230 calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapienspropionaldehydeTable II two-substrate analysis: 100 mM sodium phosphate—7 × 10⁻⁴—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KHomo sapienspropionaldehydeTable II two-substrate analysis: 100 mM sodium phosphate—0.0019—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487QHomo sapiensNAD+—0.09—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487QHomo sapienspropionaldehyde1.472 × 10⁻⁴7.3 × 10³ calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicusNAD+native enzyme isolated from rat liver mitochondria—0.004—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicusNAD+Km for propionaldehyde determined with 0.8 mM NAD+—0.004—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicusNAD+native enzyme isolated from rat liver mitochondria—0.074—pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicusNAD+Km for propionaldehyde determined with 0.8 mM NAD+—0.078—pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydeTable I assay: 50 mM sodium phosphate; 25 °C; Table I0.438 × 10⁻⁵5.4 × 10³ calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydeTable I assay: 50 mM sodium phosphate; 25 °C; Table I0.71.4 × 10⁻⁴5.0 × 10³ calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydenative enzyme isolated from rat liver mitochondria1.131 × 10⁻⁴1.1 × 10⁴ calc.pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydeKm for propionaldehyde determined with 0.8 mM NAD+1.81.5 × 10⁻⁴1.2 × 10⁴ calc.pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydeTable III/Fig. 4 burst assay: 50 mM sodium phosphate/NaOH0.7——pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)Rattus norvegicuspropionaldehydeTable III/Fig. 4 burst assay: 50 mM sodium phosphate/NaOH1.25——pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicusNAD+—0.7—pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicusNAD+—1.1—pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicuspropionaldehydeTable I assay: 50 mM sodium phosphate; 25 °C; Table I0.36 × 10⁻⁴500 calc.pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicuspropionaldehyde0.70.0017410 calc.pH 9, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicuspropionaldehydeTable III/Fig. 4 burst assay: 50 mM sodium phosphate/NaOH0.3——pH 7.4, 25 °COpen
liver mitochondrial aldehyde dehydrogenase (class 2)variant E487KRattus norvegicuspropionaldehydeTable III/Fig. 4 burst assay: 50 mM sodium phosphate/NaOH0.53——pH 7.4, 25 °COpen