Values CatLog read from this paper
Site-directed mutagenesis of beta-lactamase I: role of Glu-166
PMID 7910734doi:10.1042/bj2990671Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (54 values) from this paper: beta-lactamase I from Bacillus cereus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-lactamase I | Bacillus cereus | 6-aminopenicillanic acid | 227.4 | 1.604 | 142 | pH 7, 20 °C | Open record: beta-lactamase I, 6-aminopenicillanic acid |
| beta-lactamase I | Bacillus cereus | 6beta-furylacryloylamidopenicillanic acid | 1491 | 0.0409 | 3.64 × 10⁴ | pH 7, 20 °C | Open record: beta-lactamase I, 6beta-furylacryloylamidopenicillanic acid |
| beta-lactamase I | Bacillus cereus | benzylpenicillin | 4515 | 0.1184 | 3.81 × 10⁴ | pH 7, 20 °C | Open record: beta-lactamase I, benzylpenicillin |
| beta-lactamase I | Bacillus cereus | Nitrocefin | 49.2 | 0.0954 | 516 | pH 7, 20 °C | Open record: beta-lactamase I, Nitrocefin |
| beta-lactamase I | Bacillus cereus | phenoxymethylpenicillin | 4525 | 0.1329 | 3.41 × 10⁴ | pH 7, 20 °C | Open record: beta-lactamase I, phenoxymethylpenicillin |
| beta-lactamase Ivariant E166A | Bacillus cereus | 6-aminopenicillanic acid | — | 0.101 | — | pH 7, 20 °C | Open record: beta-lactamase I variant E166A, 6-aminopenicillanic acid |
| beta-lactamase Ivariant E166A | Bacillus cereus | 6beta-furylacryloylamidopenicillanic acid | 0.356 | 0.00559 | 63.7 | pH 7, 20 °C | Open record: beta-lactamase I variant E166A, 6beta-furylacryloylamidopenicillanic acid |
| beta-lactamase Ivariant E166A | Bacillus cereus | benzylpenicillin | 1.62 | 0.0187 | 86.6 | pH 7, 20 °C | Open record: beta-lactamase I variant E166A, benzylpenicillin |
| beta-lactamase Ivariant E166A | Bacillus cereus | Nitrocefin | 0.0151 | 0.0597 | 0.25 | pH 7, 20 °C | Open record: beta-lactamase I variant E166A, Nitrocefin |
| beta-lactamase Ivariant E166A | Bacillus cereus | phenoxymethylpenicillin | 0.923 | 0.017 | 54.4 | pH 7, 20 °C | Open record: beta-lactamase I variant E166A, phenoxymethylpenicillin |
| beta-lactamase Ivariant E166C | Bacillus cereus | 6-aminopenicillanic acid | — | 0.127 | — | pH 7, 20 °C | Open record: beta-lactamase I variant E166C, 6-aminopenicillanic acid |
| beta-lactamase Ivariant E166C | Bacillus cereus | 6beta-furylacryloylamidopenicillanic acid | 0.145 | 0.0102 | 14.2 | pH 7, 20 °C | Open record: beta-lactamase I variant E166C, 6beta-furylacryloylamidopenicillanic acid |
| beta-lactamase Ivariant E166C | Bacillus cereus | benzylpenicillin | 0.818 | 0.0184 | 44.4 | pH 7, 20 °C | Open record: beta-lactamase I variant E166C, benzylpenicillin |
| beta-lactamase Ivariant E166C | Bacillus cereus | Nitrocefin | 0.0102 | 0.0646 | 0.16 | pH 7, 20 °C | Open record: beta-lactamase I variant E166C, Nitrocefin |
| beta-lactamase Ivariant E166C | Bacillus cereus | phenoxymethylpenicillin | 0.34 | 0.0203 | 16.7 | pH 7, 20 °C | Open record: beta-lactamase I variant E166C, phenoxymethylpenicillin |
| beta-lactamase Ivariant E166Cmc | Bacillus cereus | 6-aminopenicillanic acid | — | 0.219 | — | pH 7, 20 °C | Open record: beta-lactamase I variant E166Cmc, 6-aminopenicillanic acid |
| beta-lactamase Ivariant E166Cmc | Bacillus cereus | 6beta-furylacryloylamidopenicillanic acid | 0.17 | 0.0106 | 16 | pH 7, 20 °C | Open record: beta-lactamase I variant E166Cmc, 6beta-furylacryloylamidopenicillanic acid |
| beta-lactamase Ivariant E166Cmc | Bacillus cereus | benzylpenicillin | 0.863 | 0.017 | 50.7 | pH 7, 20 °C | Open record: beta-lactamase I variant E166Cmc, benzylpenicillin |
| beta-lactamase Ivariant E166Cmc | Bacillus cereus | Nitrocefin | 0.0086 | 0.0847 | 0.1 | pH 7, 20 °C | Open record: beta-lactamase I variant E166Cmc, Nitrocefin |
| beta-lactamase Ivariant E166Cmc | Bacillus cereus | phenoxymethylpenicillin | 0.362 | 0.0103 | 35.2 | pH 7, 20 °C | Open record: beta-lactamase I variant E166Cmc, phenoxymethylpenicillin |