Values CatLog read from this paper
Studies on the kinetic mechanism of pig kidney D-amino acid oxidase by site-directed mutagenesis of tyrosine 224 and tyrosine 228
PMID 7989339doi:10.1016/s0021-9258(18)31747-2Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (42 values) from this paper: D-amino acid oxidase from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidase | Sus scrofa | D-alanineTable III | 12.7 | 2.3 | 5.5 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase, D-alanine |
| D-amino acid oxidase | Sus scrofa | D-alanineTable VII | — | — | 5.1 | pH 8.5, 25 °C | Open record: D-amino acid oxidase, D-alanine |
| D-amino acid oxidase | Sus scrofa | D-methionine | 143 | 30 | 4.8 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase, D-methionine |
| D-amino acid oxidase | Sus scrofa | D-phenylalanine | 55.9 | 12.3 | 4.54 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase, D-phenylalanine |
| D-amino acid oxidase | Sus scrofa | D-proline | 87.2 | 9.5 | 9.2 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase, D-proline |
| D-amino acid oxidase | Sus scrofa | oxygencosubstrate D-Alanine; Table III | — | 0.1 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase, oxygen |
| D-amino acid oxidase | Sus scrofa | oxygencosubstrate D-Proline; Table III | — | 1.51 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase, oxygen |
| D-amino acid oxidase | Sus scrofa | oxygencosubstrate D-Methionine; approximate Km; Table III | — | 4.2 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase, oxygen |
| D-amino acid oxidase | Sus scrofa | oxygencosubstrate D-Phenylalanine; Table III | — | 0.83 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase, oxygen |
| D-amino acid oxidase | Sus scrofa | oxygencosubstrate D-Alanine; Table VII | — | — | 130 | pH 8.5, 25 °C | Open record: D-amino acid oxidase, oxygen |
| D-amino acid oxidasevariant Y224F | Sus scrofa | D-alanineTable III | 11.6 | 2.8 | 4.1 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, D-alanine |
| D-amino acid oxidasevariant Y224F | Sus scrofa | D-alanineTable VII | — | — | 3.6 | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, D-alanine |
| D-amino acid oxidasevariant Y224F | Sus scrofa | D-methionine | 92 | 15.6 | 5.9 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, D-methionine |
| D-amino acid oxidasevariant Y224F | Sus scrofa | D-phenylalanine | 48.8 | 7.5 | 6.5 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, D-phenylalanine |
| D-amino acid oxidasevariant Y224F | Sus scrofa | D-proline | 83.7 | 9.1 | 9.2 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, D-proline |
| D-amino acid oxidasevariant Y224F | Sus scrofa | oxygencosubstrate D-Alanine; Table III | — | 0.12 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, oxygen |
| D-amino acid oxidasevariant Y224F | Sus scrofa | oxygencosubstrate D-Proline; Table III | — | 1.35 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, oxygen |
| D-amino acid oxidasevariant Y224F | Sus scrofa | oxygencosubstrate D-Methionine; Table III | — | 1.97 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, oxygen |
| D-amino acid oxidasevariant Y224F | Sus scrofa | oxygencosubstrate D-Phenylalanine; Table III | — | 0.29 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, oxygen |
| D-amino acid oxidasevariant Y224F | Sus scrofa | oxygencosubstrate D-Alanine; Table VII | — | — | 120 | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y224F, oxygen |
| D-amino acid oxidasevariant Y228F | Sus scrofa | D-alanineTable III | 5 | 27 | 0.19 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, D-alanine |
| D-amino acid oxidasevariant Y228F | Sus scrofa | D-alanineTable VII | — | — | 0.2 | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, D-alanine |
| D-amino acid oxidasevariant Y228F | Sus scrofa | D-methionine | 47.5 | 50 | 0.95 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, D-methionine |
| D-amino acid oxidasevariant Y228F | Sus scrofa | D-phenylalanine | 7.1 | 18.3 | 0.39 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, D-phenylalanine |
| D-amino acid oxidasevariant Y228F | Sus scrofa | D-proline | 11.7 | 12.2 | 0.959 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, D-proline |
| D-amino acid oxidasevariant Y228F | Sus scrofa | oxygencosubstrate D-Alanine; Table III | — | 0.05 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, oxygen |
| D-amino acid oxidasevariant Y228F | Sus scrofa | oxygencosubstrate D-Proline; Table III | — | 0.34 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, oxygen |
| D-amino acid oxidasevariant Y228F | Sus scrofa | oxygencosubstrate D-Methionine; Table III | — | 2.1 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, oxygen |
| D-amino acid oxidasevariant Y228F | Sus scrofa | oxygencosubstrate D-Phenylalanine; Table III | — | 0.2 | — | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, oxygen |
| D-amino acid oxidasevariant Y228F | Sus scrofa | oxygencosubstrate D-Alanine; Table VII | — | — | 140 | pH 8.5, 25 °C | Open record: D-amino acid oxidase variant Y228F, oxygen |