Values CatLog read from this paper

Deletion variants of L-hydroxyisocaproate dehydrogenase. Probing substrate specificity

PMID 8055963doi:10.1111/j.1432-1033.1994.tb19062.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 41 records (105 values) from this paper: L-2-hydroxyisocaproate dehydrogenase, L-2-hydroxyisocaproate dehydrogenase (L-HicDH) from Lactobacillus confusus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-2-hydroxyisocaproate dehydrogenase	Lactobacillus confusus	2-Oxocaproate	3.6 × 10⁵	0.11	3.3 × 10⁶	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenase	Lactobacillus confusus	2-Oxoisocaproate	2.4 × 10³	0.067	3.6 × 10⁴ calc.	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenase	Lactobacillus confusus	2-Phenylpyruvate	7.3 × 10³	0.026	2.8 × 10⁵	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase	Lactobacillus confusus	Pyruvate	90	3.5	26	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Asn/Pro105A/BΔ	Lactobacillus confusus	2-Oxocaproate	2.1 × 10⁴	0.62	3.4 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn/Pro105A/BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Asn/Pro105A/BΔ	Lactobacillus confusus	2-Oxoisocaproate	5.2 × 10⁵	2.8	1.9 × 10⁵	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn/Pro105A/BΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Asn/Pro105A/BΔ	Lactobacillus confusus	2-Phenylpyruvate	8.9 × 10⁴	0.33	2.7 × 10⁵	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn/Pro105A/BΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Asn/Pro105A/BΔ	Lactobacillus confusus	Pyruvate	17	27	0.63	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn/Pro105A/BΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Asn105AΔ	Lactobacillus confusus	2-Oxocaproate	4.4 × 10³	2	2.2 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn105AΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Asn105AΔ	Lactobacillus confusus	2-Oxoisocaproate	1.5 × 10⁴	0.91	1.6 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn105AΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Asn105AΔ	Lactobacillus confusus	2-Phenylpyruvate	1.9 × 10⁴	0.41	4.6 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn105AΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Asn105AΔ	Lactobacillus confusus	Pyruvate	15	25	0.6	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Asn105AΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys/Asn/Pro100A/B/105A/BΔ	Lactobacillus confusus	2-Oxocaproate	13	—	0.68	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys/Asn/Pro100A/B/105A/BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys/Asn/Pro100A/B/105A/BΔ	Lactobacillus confusus	2-Oxoisocaproate	39	43	0.91	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys/Asn/Pro100A/B/105A/BΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys/Asn/Pro100A/B/105A/BΔ	Lactobacillus confusus	2-Phenylpyruvate	1.2 × 10⁴	2.8	4.3 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys/Asn/Pro100A/B/105A/BΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys/Asn/Pro100A/B/105A/BΔ	Lactobacillus confusus	Pyruvate	0.004	30	1.3 × 10⁻⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys/Asn/Pro100A/B/105A/BΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys100A/BΔ	Lactobacillus confusus	2-Oxocaproate	20	62	0.32	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys100A/BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys100A/BΔ	Lactobacillus confusus	2-Oxoisocaproate	25	14	1.8	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys100A/BΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys100A/BΔ	Lactobacillus confusus	2-Phenylpyruvate	2.6 × 10³	3.3	790	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys100A/BΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile/Lys100A/BΔ	Lactobacillus confusus	Pyruvate	0.03	140	2.1 × 10⁻⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile/Lys100A/BΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile100AΔ	Lactobacillus confusus	2-Oxocaproate	0.63	18	0.035	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile100AΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile100AΔ	Lactobacillus confusus	2-Oxoisocaproate	1.7	18	0.092	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile100AΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Ile100AΔ	Lactobacillus confusus	2-Phenylpyruvate	8	5.8	1.4	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile100AΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Ile100AΔ	Lactobacillus confusus	Pyruvate	0.23	9.3	0.025	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Ile100AΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Leu101Δ	Lactobacillus confusus	2-Oxocaproate	—	6.4	160	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Leu101Δ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Leu101Δ	Lactobacillus confusus	2-Oxoisocaproate	4.2 × 10³	11	380	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Leu101Δ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Leu101Δ	Lactobacillus confusus	2-Phenylpyruvate	—	2.8	4.3 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Leu101Δ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Lys100BΔ	Lactobacillus confusus	2-Oxocaproate	40	5	8.0 calc.	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Lys100BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Lys100BΔ	Lactobacillus confusus	2-Oxoisocaproate	920	110	8.4	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Lys100BΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Lys100BΔ	Lactobacillus confusus	2-Phenylpyruvate	280	19	15	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Lys100BΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Lys100BΔ	Lactobacillus confusus	Pyruvate	0.76	200	0.0038	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Lys100BΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Pro105BΔ	Lactobacillus confusus	2-Oxocaproate	620	0.071	8.7 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Pro105BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Pro105BΔ	Lactobacillus confusus	2-Oxoisocaproate	490	0.17	2.9 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Pro105BΔ, 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenasevariant Pro105BΔ	Lactobacillus confusus	2-Phenylpyruvate	750	—	6.3 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Pro105BΔ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenasevariant Pro105BΔ	Lactobacillus confusus	Pyruvate	46	50	0.92	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase variant Pro105BΔ, Pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)	Lactobacillus confusus	2-Oxoisocaproate	—	—	3.6 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH), 2-Oxoisocaproate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant Ileu/Lys/Asn/Pro100A/B/105A/BΔ	Lactobacillus confusus	2-Oxocaproate	—	19	—	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant Ileu/Lys/Asn/Pro100A/B/105A/BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant Leu101Δ	Lactobacillus confusus	2-Oxocaproate	1 × 10³	—	—	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant Leu101Δ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant Leu101Δ	Lactobacillus confusus	2-Phenylpyruvate	1.2 × 10⁴	—	—	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant Leu101Δ, 2-Phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant Lys100BΔ	Lactobacillus confusus	2-Oxocaproate	—	—	8	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant Lys100BΔ, 2-Oxocaproate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant Pro105BΔ	Lactobacillus confusus	2-Phenylpyruvate	—	0.12	—	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant Pro105BΔ, 2-Phenylpyruvate