Values CatLog read from this paper
Kinetic characteristics of phosphofructokinase from Bacillus stearothermophilus: MgATP nonallosterically inhibits the enzyme
PMID 8136379doi:10.1021/bi00177a036Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (16 values) from this paper: phosphofructokinase (BsPFK) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | adenosine 5'-diphosphate | — | 0.11 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), adenosine 5'-diphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | adenosine 5'-triphosphate100 mM Tris-HCl; 10 mM MgCl2; 5 mM NH4Cl; A = MgATP | — | 0.04 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), adenosine 5'-triphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | adenosine 5'-triphosphate | — | 0.1 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), adenosine 5'-triphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | adenosine 5'-triphosphatealternative NTP substrate study | — | 0.07 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), adenosine 5'-triphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 1,6-bisphosphate | 3.2 | 0.44 | 7.3 calc. | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 1,6-bisphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphate100 mM Tris-HCl; 10 mM MgCl2; 5 mM NH4Cl; A = MgATP | 112 | 0.002 | 5.6 × 10⁴ calc. | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphatecosubstrate MgATP | — | 0.03 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphatealternative NTP substrate study; ATP fixed at 5 Km(NTP) | — | 0.033 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphatealternative NTP substrate study; GTP fixed at 5 Km(NTP) | — | 0.028 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphatealternative NTP substrate study; CTP fixed at 5 Km(NTP) | — | 0.146 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | beta-D-fructose 6-phosphatealternative NTP substrate study; UTP fixed at 5 Km(NTP) | — | 0.068 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), beta-D-fructose 6-phosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | cytidine 5'-triphosphate | — | 2.6 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), cytidine 5'-triphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | guanosine 5'-triphosphate | — | 0.18 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), guanosine 5'-triphosphate |
| phosphofructokinase (BsPFK) | Bacillus stearothermophilus | uridine 5'-triphosphate | — | 2.8 | — | pH 8.2, 30 °C | Open record: phosphofructokinase (BsPFK), uridine 5'-triphosphate |