CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of the histidine ligands to the binuclear metal center of phosphotriesterase by site-directed mutagenesis

PMID 8155644doi:10.1021/bi00180a022Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 32 records (88 values) from this paper: phosphotriesterase from Pseudomonas diminuta.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.8.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phosphotriesterasePseudomonas diminutaparaoxonTable 3—0.115 × 10⁴pH 9, 25 °COpen
phosphotriesterasePseudomonas diminutaparaoxonTable 52.3 × 10³0.0683.4 × 10⁴pH 9, 25 °COpen
phosphotriesterasePseudomonas diminutaparaoxonTable 56 × 10³0.461.3 × 10⁴pH 9, 25 °COpen
phosphotriesterasePseudomonas diminutaparaoxonTable 57.8 × 10³0.136 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H123NPseudomonas diminutaparaoxonTable 3—0.0994.9 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H123NPseudomonas diminutaparaoxonTable 52.1 × 10³0.0643.3 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H123NPseudomonas diminutaparaoxonTable 55 × 10³0.441.1 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H123NPseudomonas diminutaparaoxonTable 55.6 × 10³0.134.3 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H201NPseudomonas diminutaparaoxonTable 3—0.0091.1 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H201NPseudomonas diminutaparaoxonTable 5190.0111.7 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H201NPseudomonas diminutaparaoxonTable 51400.091.6 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H201NPseudomonas diminutaparaoxonassay supplemented with 200 µM CoCl2; Table 5250.0161.6 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H230NPseudomonas diminutaparaoxonTable 3—0.32450pH 9, 25 °COpen
phosphotriesterasevariant H230NPseudomonas diminutaparaoxonTable 5260.067390pH 9, 25 °COpen
phosphotriesterasevariant H230NPseudomonas diminutaparaoxonTable 51500.53280pH 9, 25 °COpen
phosphotriesterasevariant H230NPseudomonas diminutaparaoxonassay supplemented with 10 µM CoCl2; Table 55000.291.7 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H254NPseudomonas diminutaparaoxonTable 3—0.0419.7 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H254NPseudomonas diminutaparaoxonTable 5340.00132.6 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H254NPseudomonas diminutaparaoxonTable 53.6 × 10³0.546.7 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H254NPseudomonas diminutaparaoxonassay supplemented with 500 µM CoCl2; Table 59000.0273.3 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H257NPseudomonas diminutaparaoxonTable 3—0.436.6 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H257NPseudomonas diminutaparaoxonTable 54.4 × 10³0.558 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H257NPseudomonas diminutaparaoxonTable 53.3 × 10³0.784.2 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H257NPseudomonas diminutaparaoxonTable 57 × 10³0.641.1 × 10⁴pH 9, 25 °COpen
phosphotriesterasevariant H55NPseudomonas diminutaparaoxonTable 3—0.11180pH 9, 25 °COpen
phosphotriesterasevariant H55NPseudomonas diminutaparaoxonTable 50.830.01652pH 9, 25 °COpen
phosphotriesterasevariant H55NPseudomonas diminutaparaoxonTable 52.60.723.6pH 9, 25 °COpen
phosphotriesterasevariant H55NPseudomonas diminutaparaoxonassay supplemented with 50 µM CoCl2; Table 50.160.660.24pH 9, 25 °COpen
phosphotriesterasevariant H57NPseudomonas diminutaparaoxonTable 3—0.43490pH 9, 25 °COpen
phosphotriesterasevariant H57NPseudomonas diminutaparaoxonTable 52500.0783.2 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H57NPseudomonas diminutaparaoxonTable 53.1 × 10³1.12.8 × 10³pH 9, 25 °COpen
phosphotriesterasevariant H57NPseudomonas diminutaparaoxonassay supplemented with 80 µM CoCl2; Table 53900.311.2 × 10³pH 9, 25 °COpen