CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase

PMID 8163485doi:10.1016/s0021-9258(17)32652-2Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (28 values) from this paper: terminal deoxynucleotidyl transferase from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.31

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
terminal deoxynucleotidyl transferaseHomo sapiens(dA)50cosubstrate dATP—0.00274—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferaseHomo sapiens(dA)50cosubstrate dGTP—0.00207—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferaseHomo sapiens2'-deoxyadenosine 5'-triphosphate3.2 × 10⁴0.536.119 × 10⁴pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferaseHomo sapiens2'-deoxyguanosine 5'-triphosphate—0.12—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D343EHomo sapiens(dA)50—7 × 10⁻⁵—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D343EHomo sapiens2'-deoxyadenosine 5'-triphosphate6212.25.1pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D345EHomo sapiens(dA)50cosubstrate dATP—0.00313—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D345EHomo sapiens(dA)50cosubstrate dGTP—0.01322—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D345EHomo sapiens2'-deoxyadenosine 5'-triphosphate1.2 × 10³2.02610pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant D345EHomo sapiens2'-deoxyguanosine 5'-triphosphate—0.15—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336AHomo sapiens(dA)50cosubstrate dATP—0.00442—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336AHomo sapiens(dA)50cosubstrate dGTP—0.03618—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336AHomo sapiens2'-deoxyadenosine 5'-triphosphate2.6 × 10³3.55730pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336AHomo sapiens2'-deoxyguanosine 5'-triphosphate—1.18—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336QHomo sapiens(dA)50cosubstrate dATP—0.00561—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336QHomo sapiens(dA)50cosubstrate dGTP—0.04364—pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336QHomo sapiens2'-deoxyadenosine 5'-triphosphate1.1 × 10³4.03280pH 7.2, 35 °COpen
terminal deoxynucleotidyl transferasevariant R336QHomo sapiens2'-deoxyguanosine 5'-triphosphate—0.73—pH 7.2, 35 °COpen