Values CatLog read from this paper

Identification of basic residues involved in substrate binding and catalysis by pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii

PMID 8195200doi:10.1016/s0021-9258(17)40715-0Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (26 values) from this paper: pyrophosphate-dependent phosphofructokinase (PPi-PFK) from Propionibacterium freudenreichii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.1.90

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
pyrophosphate-dependent phosphofructokinase (PPi-PFK)	Propionibacterium freudenreichii	fructose 6-phosphate	284	0.046	6.2 × 10³ calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK), fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.0115	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK), inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant K148M	Propionibacterium freudenreichii	fructose 6-phosphate	0.058	0.119	0.49 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant K148M, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant K148M	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	1.52	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant K148M, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant K315A	Propionibacterium freudenreichii	fructose 6-phosphate	17	18	0.94 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant K315A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant K315A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.03	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant K315A, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant K315R	Propionibacterium freudenreichii	fructose 6-phosphate	288	0.05	5.8 × 10³ calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant K315R, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R186A	Propionibacterium freudenreichii	fructose 6-phosphate	21	1.97	11 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R186A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R186A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.02	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R186A, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R310A	Propionibacterium freudenreichii	fructose 6-phosphate	52	0.869	60 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R310A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R310A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.012	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R310A, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R326A	Propionibacterium freudenreichii	fructose 6-phosphate	34	31	1.1 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R326A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R326A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.065	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R326A, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R78A	Propionibacterium freudenreichii	fructose 6-phosphate	131	0.143	916 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R78A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R78A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.073	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R78A, inorganic pyrophosphate (Mg complex)
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R90A	Propionibacterium freudenreichii	fructose 6-phosphate	145	0.276	525 calc.	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R90A, fructose 6-phosphate
pyrophosphate-dependent phosphofructokinase (PPi-PFK)variant R90A	Propionibacterium freudenreichii	inorganic pyrophosphate (Mg complex)	—	0.011	—	pH 7.2, 30 °C	Open record: pyrophosphate-dependent phosphofructokinase (PPi-PFK) variant R90A, inorganic pyrophosphate (Mg complex)