Values CatLog read from this paper
The active site of yeast aspartyl-tRNA synthetase: structural and functional aspects of the aminoacylation reaction
PMID 8313877doi:10.1002/j.1460-2075.1994.tb06265.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 86 records (113 values) from this paper: aspartyl-tRNA synthetase (AspRS) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 27 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aspartyl-tRNA synthetase (AspRS) | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.03 | — | Open record: aspartyl-tRNA synthetase (AspRS), adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS) | Saccharomyces cerevisiae | L-aspartic acid | 27 | 2.5 | 11 calc. | Open record: aspartyl-tRNA synthetase (AspRS), L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS) | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.83 | — | — | Open record: aspartyl-tRNA synthetase (AspRS), yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant D342E | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.133 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant D342E, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant D342E | Saccharomyces cerevisiae | L-aspartic acid | 5.27 | 55.5 | 0.0950 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant D342E, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant D342E | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.012 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant D342E, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant D471E | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 1.75 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant D471E, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant D471E | Saccharomyces cerevisiae | L-aspartic acid | 6.09 | 19.6 | 0.311 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant D471E, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant D471E | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.0073 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant D471E, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant D471N | Saccharomyces cerevisiae | L-aspartic acid | 1.28 | 43.5 | 0.0294 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant D471N, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant E327A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.125 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E327A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant E327A | Saccharomyces cerevisiae | L-aspartic acid | 4.53 | 30 | 0.15 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant E327A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant E327A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.055 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E327A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant E327Q | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.121 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E327Q, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant E327Q | Saccharomyces cerevisiae | L-aspartic acid | 6.5 | 111 | 0.059 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant E327Q, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant E327Q | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.037 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E327Q, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant E344D | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.118 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E344D, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant E344D | Saccharomyces cerevisiae | L-aspartic acid | 21.91 | 18 | 1.2 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant E344D, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant E344D | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.19 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant E344D, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant F304A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.006 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F304A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant F304A | Saccharomyces cerevisiae | L-aspartic acid | 13.22 | 10 | 1.3 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant F304A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant F304A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.087 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F304A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant F338A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 6.66 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant F338A | Saccharomyces cerevisiae | L-aspartic acid | 3.12 | 15 | 0.21 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant F338A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant F338A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.15 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant F338H | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.555 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338H, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant F338H | Saccharomyces cerevisiae | L-aspartic acid | 16.16 | 14 | 1.2 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant F338H, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant F338H | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.15 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338H, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant F338L | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 6.45 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338L, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant F338L | Saccharomyces cerevisiae | L-aspartic acid | 0.82 | 10 | 0.082 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant F338L, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant F338L | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.1 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant F338L, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant G526A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 8.3 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant G526A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant G526A | Saccharomyces cerevisiae | L-aspartic acid | 10 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant G526A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant G528A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 3.57 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant G528A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant G528A | Saccharomyces cerevisiae | L-aspartic acid | 6.34 | 10 | 0.63 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant G528A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant G528A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.5 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant G528A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant H334K | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.29 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334K, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant H334K | Saccharomyces cerevisiae | L-aspartic acidATP-pyrophosphate exchange reaction; cosubstrate ATP | 0.62 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334K, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334K | Saccharomyces cerevisiae | L-aspartic acidtRNA aminoacylation (charging) reaction | — | 50 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334K, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334K | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.062 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334K, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant H334N | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.285 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334N, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant H334N | Saccharomyces cerevisiae | L-aspartic acidATP-pyrophosphate exchange reaction; cosubstrate ATP | 15.7 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334N, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334N | Saccharomyces cerevisiae | L-aspartic acidtRNA aminoacylation (charging) reaction | — | 150 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334N, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334N | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.15 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334N, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant H334Q | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 1 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334Q, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant H334Q | Saccharomyces cerevisiae | L-aspartic acidATP-pyrophosphate exchange reaction; cosubstrate ATP | 2.7 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334Q, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334Q | Saccharomyces cerevisiae | L-aspartic acidtRNA aminoacylation (charging) reaction | — | 4.2 × 10³ | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334Q, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334Q | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.82 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334Q, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant H334R | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.12 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334R, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant H334R | Saccharomyces cerevisiae | L-aspartic acidATP-pyrophosphate exchange reaction; cosubstrate ATP | 27 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334R, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334R | Saccharomyces cerevisiae | L-aspartic acidtRNA aminoacylation (charging) reaction | — | 50 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334R, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant H334R | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.21 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant H334R, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Q300A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.012 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant Q300A | Saccharomyces cerevisiae | L-aspartic acid | 9.83 | 7.14 | 1.38 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q300A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.035 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Q300E | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.009 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300E, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant Q300E | Saccharomyces cerevisiae | L-aspartic acid | 20.52 | 8.9 | 2.3 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300E, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q300E | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.046 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q300E, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Q303A | Saccharomyces cerevisiae | L-aspartic acid | 2.3 | 47 | 0.049 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q303A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q303E | Saccharomyces cerevisiae | L-aspartic acid | 2.08 | 56 | 0.037 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q303E, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q303N | Saccharomyces cerevisiae | L-aspartic acid | 3.11 | 166 | 0.0187 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q303N, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q307A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.105 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant Q307A | Saccharomyces cerevisiae | L-aspartic acid | 0.82 | 55.5 | 0.015 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q307A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.0029 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Q307E | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.075 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307E, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant Q307E | Saccharomyces cerevisiae | L-aspartic acid | 19.36 | 9.1 | 2.1 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307E, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Q307E | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.358 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307E, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Q307N | Saccharomyces cerevisiae | L-aspartic acid | 2.43 | 62.5 | 0.0389 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Q307N, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant R325K | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 10 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant R325K, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant R325K | Saccharomyces cerevisiae | L-aspartic acid | 10.69 | 80 | 0.13 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant R325K, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant R485H | Saccharomyces cerevisiae | L-aspartic acid | 11.02 | 30 | 0.37 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant R485H, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant S280A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.013 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S280A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant S280A | Saccharomyces cerevisiae | L-aspartic acid | 33.75 | 18 | 1.9 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant S280A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant S280A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.086 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S280A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant S301A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.0575 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S301A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant S301A | Saccharomyces cerevisiae | L-aspartic acid | 15.5 | 59 | 0.26 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant S301A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant S301A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.01 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S301A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant S329A | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.026 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S329A, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant S329A | Saccharomyces cerevisiae | L-aspartic acid | 41.44 | 3.2 | 13 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant S329A, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant S329A | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.096 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S329A, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant S481T | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.4 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S481T, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant S481T | Saccharomyces cerevisiae | L-aspartic acid | 0.27 | 14.3 | 0.019 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant S481T, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant S481T | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.13 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant S481T, yeast tRNA(Asp) | |
| aspartyl-tRNA synthetase (AspRS)variant Y456F | Saccharomyces cerevisiae | adenosine 5'-triphosphate | — | 0.027 | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Y456F, adenosine 5'-triphosphate | |
| aspartyl-tRNA synthetase (AspRS)variant Y456F | Saccharomyces cerevisiae | L-aspartic acid | 17.36 | 25 | 0.69 calc. | Open record: aspartyl-tRNA synthetase (AspRS) variant Y456F, L-aspartic acid | |
| aspartyl-tRNA synthetase (AspRS)variant Y456F | Saccharomyces cerevisiae | yeast tRNA(Asp) | 0.44 | — | — | Open record: aspartyl-tRNA synthetase (AspRS) variant Y456F, yeast tRNA(Asp) |