CatLogenzyme kinetics catalog

Values CatLog read from this paper

Role of Ser-238 and Lys-240 in the hydrolysis of third-generation cephalosporins by SHV-type beta-lactamases probed by site-directed mutagenesis and three-dimensional modeling

PMID 8429044doi:10.1016/s0021-9258(18)53748-0Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (28 values) from this paper: SHV-2 beta-lactamase, SHV-1 beta-lactamase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.2.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
SHV-1 beta-lactamase—ampicillin—0.094—pH 7, 25 °COpen
SHV-1 beta-lactamase—cephaloridine—0.379—pH 7, 25 °COpen
SHV-1 beta-lactamase—cephalothin—0.062—pH 7, 25 °COpen
SHV-1 beta-lactamase—nitrocefin—0.638—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant E240K—ampicillin—0.087—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant E240K—cephaloridine—0.269—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant E240K—cephalothin—0.03—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant E240K—nitrocefin—0.373—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant G238S—ampicillin—0.031—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant G238S—cefotaxime—0.014—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant G238S—cephaloridine—0.045—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant G238S—cephalothin—0.0072—pH 7, 25 °COpen
SHV-1 beta-lactamasevariant G238S—nitrocefin—0.148—pH 7, 25 °COpen
SHV-2 beta-lactamase—ampicillin—0.035—pH 7, 25 °COpen
SHV-2 beta-lactamase—cefotaxime—0.013—pH 7, 25 °COpen
SHV-2 beta-lactamase—cephaloridine—0.044—pH 7, 25 °COpen
SHV-2 beta-lactamase—cephalothin—0.0091—pH 7, 25 °COpen
SHV-2 beta-lactamase—nitrocefin—0.194—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—ampicillin—0.015—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—cefotaxime—0.015—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—ceftazidime—0.035—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—cephaloridine—0.049—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—cephalothin—0.01—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant E240K—nitrocefin—0.14—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant S238G—ampicillin—0.065—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant S238G—cephaloridine—0.474—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant S238G—cephalothin—0.051—pH 7, 25 °COpen
SHV-2 beta-lactamasevariant S238G—nitrocefin—0.416—pH 7, 25 °COpen