Values CatLog read from this paper
Inversion of the substrate specificity of yeast alcohol dehydrogenase
PMID 8463307doi:10.1016/s0021-9258(18)53028-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 102 records (266 values) from this paper: alcohol dehydrogenase I (ScADH) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | (R)-2-butanol | 0.05 | 61 | 8 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), (R)-2-butanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | (S)-2-butanol | 1 | 55 | 0.018 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), (S)-2-butanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | 1-heptanol | 14 | 7.8 | 1.8 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), 1-heptanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | 1-nonanol | 8.6 | 1.7 | 5.1 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), 1-nonanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | 1-octanol | 17 | 5.3 | 3.3 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), 1-octanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | 2-methyl-1-propanol | 0.19 | 25 | 0.0076 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), 2-methyl-1-propanol |
| alcohol dehydrogenase I (ScADH) | Saccharomyces cerevisiae | 2-propanol | 4.8 | 190 | 0.025 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH), 2-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | (R)-2-butanol | 0.11 | 89 | 0.0012 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, (R)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | (RS)-2-methyl-1-butanol | 0.13 | 31 | 0.0042 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, (RS)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | (S)-2-butanol | 1.3 | 48 | 0.027 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, (S)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | (S)-2-methyl-1-butanol | 0.14 | 51 | 0.0027 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, (S)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 1-butanol | 69 | 52 | 1.3 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 1-hexanol | 20 | 42 | 0.48 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 1-hexanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 1-pentanol | 13 | 40 | 0.33 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 1-pentanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 1-propanol | 56 | 22 | 2.5 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 1-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 2-methyl-1-propanol | 1.7 | 42 | 0.04 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 2-methyl-1-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | 2-propanol | 4 | 170 | 0.024 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, 2-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | acetaldehyde | 1.5 × 10³ | 0.74 | 2.0 × 10³ calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, acetaldehyde |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | benzyl alcohol | 0.4 | 52 | 0.014 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, benzyl alcohol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | ethanolfull steady-state kinetic analysis | 200 | 17 | 12 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | ethanol | 210 | — | — | 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | ethanolNAD+ fixed at 2 mM; alcohol varied (Table IV) | 200 | 17 | 12 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | NAD+ | — | 0.091 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, NAD+ |
| alcohol dehydrogenase I (ScADH)variant T48S | Saccharomyces cerevisiae | NADH | — | 0.11 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S, NADH |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | (R)-2-butanol | 0.047 | 94 | 5 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, (R)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | (RS)-2-methyl-1-butanol | 0.14 | 60 | 0.0023 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, (RS)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | (S)-2-butanol | 0.33 | 79 | 0.0042 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, (S)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | (S)-2-methyl-1-butanol | 0.18 | 54 | 0.0033 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, (S)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-butanol | 4.1 | 25 | 0.16 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-heptanol | 4 | 8.6 | 0.46 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-heptanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-hexanol | 3 | 35 | 0.09 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-hexanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-nonanol | 3.8 | 4.1 | 0.93 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-nonanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-octanol | 3.1 | 3.8 | 0.82 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-octanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-pentanol | 1.7 | 19 | 0.09 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-pentanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 1-propanol | 9.3 | 68 | 0.14 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 1-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 2-methyl-1-propanol | 0.12 | 32 | 0.0038 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 2-methyl-1-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 2-propanol | 0.15 | 190 | 7.9 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 2-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | 3-methyl-1-butanol | 0.12 | 44 | 0.0027 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, 3-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | benzyl alcohol | 0.12 | 13 | 0.0092 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, benzyl alcohol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | cyclohexanol | 0.009 | 52 | 1.7 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, cyclohexanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | ethanolfull steady-state kinetic analysis | 120 | 160 | 0.75 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | ethanol | 10 | — | — | 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | ethanolNAD+ fixed at 10 mM; alcohol varied (Table IV) | 60 | 114 | 0.53 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W57M/W93A | Saccharomyces cerevisiae | NAD+ | — | 5.4 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W57M/W93A, NAD+ |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | (R)-2-butanol | 0.019 | 135 | 1.4 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, (R)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | (RS)-2-methyl-1-butanol | 1.2 | 21 | 0.057 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, (RS)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | (S)-2-butanol | 0.19 | 870 | 2.2 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, (S)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | (S)-2-methyl-1-butanol | 0.34 | 21 | 0.016 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, (S)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-butanol | 8.4 | 36 | 0.22 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-heptanol | 105 | 3.1 | 34 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-heptanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-hexanol | 75 | 6 | 16 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-hexanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-nonanol | 49 | 1.2 | 38 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-nonanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-octanol | 71 | 1.3 | 54 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-octanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-pentanol | 39 | 15 | 3.5 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-pentanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 1-propanol | 17 | 410 | 0.043 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 1-propanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | 3-methyl-1-butanol | 6 | 26 | 0.23 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, 3-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | acetaldehyde | 530 | 93 | 5.7 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, acetaldehyde |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | benzyl alcohol | 0.014 | 2.8 | 0.005 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, benzyl alcohol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | butanol | 7.8 | 33 | 0.24 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, butanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | butyraldehyde | 120 | 3.9 | 31 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, butyraldehyde |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | cyclohexanol | 0.045 | 40 | 0.0011 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, cyclohexanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | ethanolfull steady-state kinetic analysis | 140 | 4.2 × 10³ | 0.033 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | ethanol | 16 | — | — | 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | ethanolNAD+ fixed at 5 mM; alcohol varied (Table IV) | 110 | 2.9 × 10³ | 0.037 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | NAD+ethanol/NAD+ forward and acetaldehyde/NADH reverse (Table II | — | 0.92 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, NAD+ |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | NAD+T48S:W93A (Butanol) column); cosubstrate butanol | — | 0.59 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, NAD+ |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | NADHethanol/NAD+ forward and acetaldehyde/NADH reverse (Table II | — | 0.13 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, NADH |
| alcohol dehydrogenase I (ScADH)variant T48S/W93A | Saccharomyces cerevisiae | NADHT48S:W93A (Butanol) column); cosubstrate butyraldehyde | — | 0.039 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant T48S/W93A, NADH |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | (R)-2-butanol | 0.36 | 250 | 0.0014 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, (R)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | (S)-2-butanol | 0.43 | 130 | 0.0033 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, (S)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 1-butanol | 14 | 41 | 0.34 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 1-butanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 1-hexanol | 7.5 | 16 | 0.07 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 1-hexanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 1-pentanol | 5.9 | 13 | 0.45 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 1-pentanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 1-propanol | 17 | 65 | 0.26 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 1-propanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 2-methyl-1-propanol | 0.17 | 68 | 0.0025 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 2-methyl-1-propanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | 2-propanol | 2.5 | 210 | 0.012 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, 2-propanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | acetaldehyde | 1.9 × 10³ | 3.7 | 510 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, acetaldehyde |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | ethanolfull steady-state kinetic analysis | 220 | 45 | 4.9 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, ethanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | ethanol | 94 | — | — | 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, ethanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | ethanolNAD+ fixed at 10 mM; alcohol varied (Table IV) | 220 | 45 | 5 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, ethanol |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | NAD+ | — | 0.83 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, NAD+ |
| alcohol dehydrogenase I (ScADH)variant W57M | Saccharomyces cerevisiae | NADH | — | 0.28 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W57M, NADH |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | (R)-2-butanol | 0.028 | 46 | 6.1 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, (R)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | (RS)-2-methyl-1-butanol | 0.4 | 43 | 0.0093 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, (RS)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | (S)-2-butanol | 0.027 | 55 | 4.9 × 10⁻⁴ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, (S)-2-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | (S)-2-methyl-1-butanol | 0.16 | 58 | 0.0028 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, (S)-2-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-butanol | 7.8 | 51 | 0.15 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-heptanol | 140 | 5.5 | 26 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-heptanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-hexanol | 44 | 9 | 5.1 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-hexanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-nonanol | 130 | 10 | 13 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-nonanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-octanol | 55 | 1.9 | 29 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-octanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-pentanol | 20 | 13 | 0.5 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-pentanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 1-propanol | 9.1 | 150 | 0.061 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 1-propanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 2-methyl-1-propanol | 0.34 | 110 | 0.0031 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 2-methyl-1-propanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 2-propanol | 1.7 | 1.1 × 10³ | 0.0015 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 2-propanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | 3-methyl-1-butanol | 2.4 | 46 | 0.052 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, 3-methyl-1-butanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | benzyl alcohol | 0.26 | 15 | 0.017 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, benzyl alcohol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | cyclohexanol | 0.018 | 43 | 4.1 × 10⁻⁵ | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, cyclohexanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | ethanolfull steady-state kinetic analysis | 110 | 1.6 × 10³ | 0.069 calc. | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | ethanol | 30 | — | — | 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | ethanolNAD+ fixed at 5 mM; alcohol varied (Table IV) | 66 | 1.17 × 10³ | 0.057 | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, ethanol |
| alcohol dehydrogenase I (ScADH)variant W93A | Saccharomyces cerevisiae | NAD+ | — | 2.3 | — | pH 7.3, 30 °C | Open record: alcohol dehydrogenase I (ScADH) variant W93A, NAD+ |