Values CatLog read from this paper
Kinetic mechanisms of the A and B isozymes of O-acetylserine sulfhydrylase from Salmonella typhimurium LT-2 using the natural and alternative reactants
PMID 8518286doi:10.1021/bi00076a017Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (40 values) from this paper: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), O-acetylserine sulfhydrylase-B isozyme B (OASS-B) from Salmonella typhimurium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | 5-mercapto-2-nitrobenzoic acidcosubstrate OAS | — | 0.6 | 0.95 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), 5-mercapto-2-nitrobenzoic acid |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | 5-mercapto-2-nitrobenzoic acidcosubstrate BCA | — | 0.03 | 0.59 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), 5-mercapto-2-nitrobenzoic acid |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | beta-chloro-L-alaninecomputer-assisted sulfide ion selective electrode assay | 2 | 0.5 | 3.7 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), beta-chloro-L-alanine |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | beta-chloro-L-alanineTNB disappearance followed at 412 nm; 100 mM Hepes | 0.016 | 13 | 0.0012 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), beta-chloro-L-alanine |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | O-acetyl-L-serinecomputer-assisted sulfide ion selective electrode assay | 130 | 1 | 140 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | O-acetyl-L-serineTNB disappearance followed at 412 nm; 100 mM Hepes | 0.56 | 15 | 0.037 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | sodium sulfidecosubstrate OAS | — | 0.006 | 2.4 × 10⁴ | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), sodium sulfide |
| O-acetylserine sulfhydrylase-A isozyme A (OASS-A) | Salmonella typhimurium | sodium sulfidecosubstrate BCA | — | 5 × 10⁻⁴ | 3.9 × 10³ | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-A isozyme A (OASS-A), sodium sulfide |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | 5-mercapto-2-nitrobenzoic acidcosubstrate OAS | — | 0.43 | 18 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), 5-mercapto-2-nitrobenzoic acid |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | 5-mercapto-2-nitrobenzoic acidcosubstrate BCA | — | 0.2 | 32 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), 5-mercapto-2-nitrobenzoic acid |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | beta-chloro-L-alaninecomputer-assisted sulfide ion selective electrode assay | 22 | 2.2 | 10 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), beta-chloro-L-alanine |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | beta-chloro-L-alanineTNB disappearance followed at 412 nm; 100 mM Hepes | 7 | 0.5 | 12 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), beta-chloro-L-alanine |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | O-acetyl-L-serinecomputer-assisted sulfide ion selective electrode assay | 115 | 0.9 | 130 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | O-acetyl-L-serineTNB disappearance followed at 412 nm; 100 mM Hepes | 8 | 0.19 | 41 | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), O-acetyl-L-serine |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | sodium sulfidecosubstrate OAS | — | 0.01 | 1.2 × 10⁴ | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), sodium sulfide |
| O-acetylserine sulfhydrylase-B isozyme B (OASS-B) | Salmonella typhimurium | sodium sulfidecosubstrate BCA | — | 0.012 | 1.8 × 10³ | pH 7, 25 °C | Open record: O-acetylserine sulfhydrylase-B isozyme B (OASS-B), sodium sulfide |