Values CatLog read from this paper
Engineering yeast alcohol dehydrogenase. Replacing Trp54 by Leu broadens substrate specificity
PMID 8532667doi:10.1093/protein/8.5.457Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (58 values) from this paper: alcohol dehydrogenase (Adh 1) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | (S)-2-butanol | 11.9 | 93.3 | 0.127 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), (S)-2-butanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 1-butanol | 118 | 53.9 | 2.2 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 1-butanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 1-hexanol | 29.6 | 13.8 | 2.14 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 1-hexanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 1-pentanol | 56.2 | 56.8 | 0.99 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 1-pentanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 1-propanol | 123 | 13.6 | 9.06 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 1-propanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 2-Propanol | 53.1 | 268 | 0.198 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 2-Propanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | 4-Methyl-1-pentanol | 6.7 | 53.8 | 0.115 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), 4-Methyl-1-pentanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | acetaldehyde | 932 | — | — | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), acetaldehyde |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | Cinnamyl alcohol | 133 | 4.58 | 29 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), Cinnamyl alcohol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | ethanol | 308 | 4.03 | 76.3 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), ethanol |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | NAD+ | — | 0.211 | — | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), NAD+ |
| alcohol dehydrogenase (Adh 1) | Saccharomyces cerevisiae | NADH | — | 0.123 | — | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1), NADH |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 1-butanol | 32.7 | 29.4 | 1.11 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 1-butanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 1-hexanol | 23.9 | 6.5 | 3.67 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 1-hexanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 1-pentanol | 18.4 | 11.8 | 1.56 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 1-pentanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 1-propanol | 28.7 | 43.9 | 0.65 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 1-propanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 2-Propanol | 5.8 | 193 | 0.03 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 2-Propanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | 4-Methyl-1-pentanol | 18.6 | 6.7 | 2.79 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, 4-Methyl-1-pentanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | Cinnamyl alcohol | 98 | 1.5 | 67.3 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, Cinnamyl alcohol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | Ethanol | 102.1 | 13.4 | 7.64 | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, Ethanol |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | NAD+ | 99.2 | 1.09 | 91.0 calc. | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, NAD+ |
| alcohol dehydrogenase (Adh 1)variant W54L | Saccharomyces cerevisiae | NADH | 211 | 0.094 | 2.2 × 10³ calc. | pH 8.2, 25 °C | Open record: alcohol dehydrogenase (Adh 1) variant W54L, NADH |