Values CatLog read from this paper
Profactor IX propeptide and glutamate substrate binding sites on the vitamin K-dependent carboxylase identified by site-directed mutagenesis
PMID 8663364doi:10.1074/jbc.271.30.17837Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 33 records (97 values) from this paper: vitamin K-dependent gamma-glutamyl carboxylase from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys | 0.02 | 0.0023 | 8.7 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0; 0.16% CHAPS; 0.16% phosphatidylcholine | 0.7 | 1.1 | 0.64 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0; 0.16% CHAPS; 0.16% phosphatidylcholine | 0.059 | 2.01 | 0.029 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0; 0.16% CHAPS; 0.16% phosphatidylcholine | 0.324 | 0.91 | 0.36 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0; 0.16% CHAPS; 0.16% phosphatidylcholine | 0.465 | 0.78 | 0.6 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0; 0.16% CHAPS; 0.16% phosphatidylcholine | 0.488 | 0.78 | 0.63 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0 assay with 14CO2 incorporation | 0.7 | 1 | 0.70 calc. | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | Phe-Leu-Glu-Glu-Leu25 mM MOPS pH 7.0 assay with 14CO2 incorporation | 0.6 | 1.5 | 0.40 calc. | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | vitamin K1 hydroquinone3.6 mM FLEEL; 16 µM proFIX18; 0.8 M ammonium sulfate | 0.66 | 0.074 | 8.9 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylase | Bos taurus | vitamin K1 hydroquinone5 µM proPT28; cosubstrate proPT28 | 0.02 | 0.004 | 5 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys | 0.06 | 0.0081 | 7.4 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | Phe-Leu-Glu-Glu-Leu16 µM proFIX18 | 0.64 | 1.3 | 0.49 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | Phe-Leu-Glu-Glu-Leu0 µM proFIX18 | 0.01 | 2.03 | 0.005 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | Phe-Leu-Glu-Glu-Leu0.16 µM proFIX18 | 0.175 | 1.54 | 0.11 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | Phe-Leu-Glu-Glu-Leu1.6 µM proFIX18 | 0.391 | 1.25 | 0.31 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | Phe-Leu-Glu-Glu-Leu160 µM proFIX18 | 0.645 | 0.92 | 0.7 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | vitamin K1 hydroquinone3.6 mM FLEEL; 16 µM proFIX18; 0.8 M ammonium sulfate | 0.6 | 0.097 | 6.2 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R234A/H235A | Bos taurus | vitamin K1 hydroquinone5 µM proPT28; cosubstrate proPT28 | 0.033 | 0.007 | 4.7 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R234A/H235A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R359A/H360A/K361A | Bos taurus | His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys | 0.002 | 0.0041 | 0.49 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R359A/H360A/K361A, His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R359A/H360A/K361A | Bos taurus | Phe-Leu-Glu-Glu-Leu | 0.19 | 1.5 | 0.13 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R359A/H360A/K361A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R359A/H360A/K361A | Bos taurus | vitamin K1 hydroquinone | 0.11 | 0.043 | 2.6 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R359A/H360A/K361A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys | 0.042 | 0.0052 | 7.7 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | Phe-Leu-Glu-Glu-Leu16 µM proFIX18 | 0.72 | 1.6 | 0.45 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | Phe-Leu-Glu-Glu-Leu0 µM proFIX18 | 0.015 | 2.06 | 0.007 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | Phe-Leu-Glu-Glu-Leu0.16 µM proFIX18 | 0.153 | 1.78 | 0.09 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | Phe-Leu-Glu-Glu-Leu1.6 µM proFIX18 | 0.347 | 1.23 | 0.28 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | Phe-Leu-Glu-Glu-Leu160 µM proFIX18 | 0.56 | 1.01 | 0.55 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | vitamin K1 hydroquinone3.6 mM FLEEL; 16 µM proFIX18; 0.8 M ammonium sulfate | 0.56 | 0.053 | 11 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R406A/H408A | Bos taurus | vitamin K1 hydroquinone5 µM proPT28; cosubstrate proPT28 | 0.025 | 0.0038 | 6.6 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R406A/H408A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R513A/K515A | Bos taurus | His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys | 0.056 | 0.0144 | 3.9 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R513A/K515A, His-Val-Phe-Leu-Ala-Pro-Gln-Gln-Ala-Arg-Ser-Leu-Leu-Gln-Arg-Val-Arg-Arg-Ala-Asn-Thr-Phe-Leu-Glu-Glu-Val-Arg-Lys |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R513A/K515A | Bos taurus | Phe-Leu-Glu-Glu-Leu | 0.07 | 1.9 | 0.04 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R513A/K515A, Phe-Leu-Glu-Glu-Leu |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R513A/K515A | Bos taurus | vitamin K1 hydroquinone3.6 mM FLEEL; 16 µM proFIX18; 0.8 M ammonium sulfate | 0.1 | 0.032 | 3.1 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R513A/K515A, vitamin K1 hydroquinone |
| vitamin K-dependent gamma-glutamyl carboxylasevariant R513A/K515A | Bos taurus | vitamin K1 hydroquinone5 µM proPT28; cosubstrate proPT28 | 0.018 | 0.0123 | 1.5 | pH 7, 25 °C | Open record: vitamin K-dependent gamma-glutamyl carboxylase variant R513A/K515A, vitamin K1 hydroquinone |