Values CatLog read from this paper
Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases
PMID 8665867doi:10.1002/j.1460-2075.1996.tb00617.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (18 values) from this paper: poly(A) polymerase from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| poly(A) polymerase | Bos taurus | oligo(A)11-20 | 21.1 | 8 × 10⁻⁵ | 2.6 × 10⁵ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase, oligo(A)11-20 |
| poly(A) polymerasevariant 1Met-481 | Bos taurus | oligo(A)11-20 | 0.096 | 0.00154 | 62 calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant 1Met-481, oligo(A)11-20 |
| poly(A) polymerasevariant 1Met-488 | Bos taurus | oligo(A)11-20 | 5.5 | 0.0055 | 1.0 × 10³ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant 1Met-488, oligo(A)11-20 |
| poly(A) polymerasevariant 1Met-503 | Bos taurus | oligo(A)11-20 | 10.1 | 0.0025 | 4.0 × 10³ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant 1Met-503, oligo(A)11-20 |
| poly(A) polymerasevariant 1Met-531 | Bos taurus | oligo(A)11-20 | 23.3 | 6 × 10⁻⁵ | 3.9 × 10⁵ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant 1Met-531, oligo(A)11-20 |
| poly(A) polymerasevariant D113A | Bos taurus | oligo(A)11-20 | 0.029 | — | — | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D113A, oligo(A)11-20 |
| poly(A) polymerasevariant D113A/D115A (DID 113-115 AIA) | Bos taurus | oligo(A)11-20 | 0.065 | — | — | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D113A/D115A (DID 113-115 AIA), oligo(A)11-20 |
| poly(A) polymerasevariant D115A | Bos taurus | oligo(A)11-20 | 0.016 | — | — | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D115A, oligo(A)11-20 |
| poly(A) polymerasevariant D167A | Bos taurus | oligo(A)11-20 | 0.014 | — | — | pH 8.3, 37 °C | Open record: poly(A) polymerase variant D167A, oligo(A)11-20 |
| poly(A) polymerasevariant K504A/K505A/K506A/K507A (K4504-508A4) | Bos taurus | oligo(A)11-20 | 24.6 | 0.00185 | 1.33 × 10⁴ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant K504A/K505A/K506A/K507A (K4504-508A4), oligo(A)11-20 |
| poly(A) polymerasevariant K507T/H508D (KH507-508TD) | Bos taurus | oligo(A)11-20 | 25.1 | 4.8 × 10⁻⁴ | 5.2 × 10⁴ calc. | pH 8.3, 37 °C | Open record: poly(A) polymerase variant K507T/H508D (KH507-508TD), oligo(A)11-20 |