Values CatLog read from this paper
Protein engineering studies of dichloromethane dehalogenase/glutathione S-transferase from Methylophilus sp. strain DM11. Ser12 but not Tyr6 is required for enzyme activity
PMID 8706748doi:10.1111/j.1432-1033.1996.0410u.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (23 values) from this paper: dichloromethane dehalogenase/glutathione S-transferase from Methylophilus sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dichloromethane dehalogenase/glutathione S-transferase | Methylophilus sp. | dibromomethaneapparent kcat; kcat per subunit; apparent Km | 5.5 | 0.0035 | 1.6 × 10³ calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferase | Methylophilus sp. | dibromomethaneapparent kcat/Km | — | — | 1.6 × 10³ | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferase | Methylophilus sp. | dichloromethaneapparent kcat; kcat per subunit; apparent Km | 3.3 | 0.059 | 56 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase, dichloromethane |
| dichloromethane dehalogenase/glutathione S-transferase | Methylophilus sp. | dichloromethaneapparent kcat/Km | — | — | 56 | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase, dichloromethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant H8F | Methylophilus sp. | dibromomethane | 3.4 | 0.001 | 3.4 × 10³ calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant H8F, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant H8F | Methylophilus sp. | dichloromethane | 3.6 | 0.049 | 73 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant H8F, dichloromethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant H8Y | Methylophilus sp. | dibromomethane | 3 | 0.0032 | 940 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant H8Y, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant H8Y | Methylophilus sp. | dichloromethane | 3 | 0.055 | 55 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant H8Y, dichloromethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant S12T | Methylophilus sp. | dibromomethane | 6 × 10⁻⁴ | — | — | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant S12T, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant Y6A | Methylophilus sp. | dibromomethane | 4.4 | 5 × 10⁻⁴ | 8.8 × 10³ calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant Y6A, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant Y6A | Methylophilus sp. | dichloromethane | 2.3 | 0.034 | 68 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant Y6A, dichloromethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant Y6F | Methylophilus sp. | dibromomethane | 5.6 | 0.0029 | 1.9 × 10³ calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant Y6F, dibromomethane |
| dichloromethane dehalogenase/glutathione S-transferasevariant Y6F | Methylophilus sp. | dichloromethane | 2.6 | 0.032 | 81 calc. | pH 8.5, 30 °C | Open record: dichloromethane dehalogenase/glutathione S-transferase variant Y6F, dichloromethane |