Values CatLog read from this paper

Conversion of L-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine

PMID 8910450doi:10.1074/jbc.271.45.28300Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (28 values) from this paper: L-lactate oxidase from Aerococcus viridans.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-lactate oxidase	Aerococcus viridans	DL-glyceric acid	0.87	5	0.17 calc.	pH 7, 25 °C	Open record: L-lactate oxidase, DL-glyceric acid
L-lactate oxidase	Aerococcus viridans	DL-α-hydroxy-n-butyric acid	11	18	0.61 calc.	pH 7, 25 °C	Open record: L-lactate oxidase, DL-α-hydroxy-n-butyric acid
L-lactate oxidase	Aerococcus viridans	DL-α-hydroxy-n-valeric acid	1.25	5.5	0.23 calc.	pH 7, 25 °C	Open record: L-lactate oxidase, DL-α-hydroxy-n-valeric acid
L-lactate oxidase	Aerococcus viridans	L-lactic acid	280	0.87	330 calc.	pH 7, 25 °C	Open record: L-lactate oxidase, L-lactic acid
L-lactate oxidase	Aerococcus viridans	L-mandelic acid	0.0033	0.3	0.011 calc.	pH 7, 25 °C	Open record: L-lactate oxidase, L-mandelic acid
L-lactate oxidase	Aerococcus viridans	oxygencosubstrate L-Lactate	—	0.23	—	pH 7, 25 °C	Open record: L-lactate oxidase, oxygen
L-lactate oxidase	Aerococcus viridans	oxygencosubstrate DL-α-OH-n-Butyrate	—	1.7	—	pH 7, 25 °C	Open record: L-lactate oxidase, oxygen
L-lactate oxidasevariant A95G	Aerococcus viridans	DL-glyceric acid	31.3	53	0.59 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, DL-glyceric acid
L-lactate oxidasevariant A95G	Aerococcus viridans	DL-α-hydroxy-n-butyric acid	510	27	19 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, DL-α-hydroxy-n-butyric acid
L-lactate oxidasevariant A95G	Aerococcus viridans	DL-α-hydroxy-n-valeric acid	52	10	5.2 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, DL-α-hydroxy-n-valeric acid
L-lactate oxidasevariant A95G	Aerococcus viridans	L-lactic acid	250	6.75	37 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, L-lactic acid
L-lactate oxidasevariant A95G	Aerococcus viridans	L-mandelic acid	15.8	20	0.79 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, L-mandelic acid
L-lactate oxidasevariant A95G	Aerococcus viridans	L-α-hydroxy-iso-valeric acid	8.3	125	0.067 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, L-α-hydroxy-iso-valeric acid
L-lactate oxidasevariant A95G	Aerococcus viridans	L-α-hydroxy-β-methylvaleric acid	11.2	140	0.080 calc.	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, L-α-hydroxy-β-methylvaleric acid
L-lactate oxidasevariant A95G	Aerococcus viridans	oxygencosubstrate L-Lactate	—	1.1	—	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, oxygen
L-lactate oxidasevariant A95G	Aerococcus viridans	oxygencosubstrate DL-α-OH-n-Butyrate	—	2.14	—	pH 7, 25 °C	Open record: L-lactate oxidase variant A95G, oxygen