Values CatLog read from this paper
Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue
PMID 8939691doi:10.1016/s1074-5521(96)90251-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (23 values) from this paper: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H123A | — | adenosine 5'-triphosphate | 0.571 | 0.0116 | 49.2 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H123A, adenosine 5'-triphosphate | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H123A | — | Amikacin | 0.87 | — | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H123A, Amikacin | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H123A | — | Kanamycin A | 0.49 | 9.2 × 10⁻⁴ | 530 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H123A, Kanamycin A | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H123A | — | Neomycin B | 0.83 | 2.9 × 10⁻⁴ | 2.9 × 10³ calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H123A, Neomycin B | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H188A | — | adenosine 5'-triphosphate | — | 0.0335 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H188A, adenosine 5'-triphosphate | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H188A | — | Amikacin | — | 0.184 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H188A, Amikacin | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H188A | — | Kanamycin A | — | 0.0174 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H188A, Kanamycin A | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H188A | — | Neomycin B | — | 0.0032 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H188A, Neomycin B | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H78A | — | adenosine 5'-triphosphate | 0.66 | 0.0109 | 61 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H78A, adenosine 5'-triphosphate | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H78A | — | Amikacin | 1.74 | 0.206 | 8.45 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H78A, Amikacin | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H78A | — | Kanamycin A | 0.67 | 0.0051 | 130 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H78A, Kanamycin A | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H78A | — | Neomycin B | 2.46 | 0.027 | 91 calc. | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H78A, Neomycin B | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H82A | — | adenosine 5'-triphosphate | — | 0.01217 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H82A, adenosine 5'-triphosphate | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H82A | — | Amikacin | — | 0.0931 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H82A, Amikacin | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H82A | — | Kanamycin A | — | 0.00244 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H82A, Kanamycin A | |
| aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa)variant H82A | — | Neomycin B | — | 0.00131 | — | Open record: aminoglycoside 3'-phosphotransferase type IIIa (APH(3')-IIIa) variant H82A, Neomycin B |