Values CatLog read from this paper

Substrate specificity and identification of functional groups of homoserine kinase from Escherichia coli

PMID 8973190doi:10.1021/bi962203zOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 38 records (74 values) from this paper: homoserine kinase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.1.39

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
homoserine kinase	Escherichia coli	ATP	0.016	—	—		Open record: homoserine kinase, ATP
homoserine kinase	Escherichia coli	ATPcosubstrate L-homoserine	—	0.13	—		Open record: homoserine kinase, ATP
homoserine kinase	Escherichia coli	D-homoserine	8.3	31.8	0.26		Open record: homoserine kinase, D-homoserine
homoserine kinase	Escherichia coli	L-2-amino-1,4-butanediol	2	11.6	0.17		Open record: homoserine kinase, L-2-amino-1,4-butanediol
homoserine kinase	Escherichia coli	L-2-amino-5-hydroxyvalerate	2.5	1.1	2.3		Open record: homoserine kinase, L-2-amino-5-hydroxyvalerate
homoserine kinase	Escherichia coli	L-aspartate β-semialdehyde	2.1	0.28	7.5		Open record: homoserine kinase, L-aspartate β-semialdehyde
homoserine kinase	Escherichia coli	L-homoserine	18.3	0.14	184		Open record: homoserine kinase, L-homoserine
homoserine kinase	Escherichia coli	L-homoserine ethyl ester	13.6	1.9	7.2		Open record: homoserine kinase, L-homoserine ethyl ester
homoserine kinase	Escherichia coli	L-homoserine isobutyl ester	16.4	6.9	2.4		Open record: homoserine kinase, L-homoserine isobutyl ester
homoserine kinase	Escherichia coli	L-homoserine isopropyl ester	13.6	1.2	11.3		Open record: homoserine kinase, L-homoserine isopropyl ester
homoserine kinase	Escherichia coli	L-homoserine methyl ester	14.7	4.9	3		Open record: homoserine kinase, L-homoserine methyl ester
homoserine kinase	Escherichia coli	L-homoserine n-butyl ester	29.1	5.8	5		Open record: homoserine kinase, L-homoserine n-butyl ester
homoserine kinase	Escherichia coli	L-homoserine n-propyl ester	14	3.5	4		Open record: homoserine kinase, L-homoserine n-propyl ester
homoserine kinasevariant H139L	Escherichia coli	ATP	2.51	0.49	5.1 calc.		Open record: homoserine kinase variant H139L, ATP
homoserine kinasevariant H139L	Escherichia coli	L-homoserine	0.52	—	—	pH 8	Open record: homoserine kinase variant H139L, L-homoserine
homoserine kinasevariant H202L	Escherichia coli	ATP	0.071	—	—		Open record: homoserine kinase variant H202L, ATP
homoserine kinasevariant H202L	Escherichia coli	ATPcosubstrate L-homoserine	—	0.11	—		Open record: homoserine kinase variant H202L, ATP
homoserine kinasevariant H202L	Escherichia coli	L-homoserineTable 2	9.06	0.11	82 calc.		Open record: homoserine kinase variant H202L, L-homoserine
homoserine kinasevariant H202L	Escherichia coli	L-homoserineTable 3	—	—	82.7		Open record: homoserine kinase variant H202L, L-homoserine
homoserine kinasevariant H202L	Escherichia coli	L-homoserine butyl ester	2.5	—	2.3		Open record: homoserine kinase variant H202L, L-homoserine butyl ester
homoserine kinasevariant H202L	Escherichia coli	L-homoserine ethyl ester	4.1	—	3		Open record: homoserine kinase variant H202L, L-homoserine ethyl ester
homoserine kinasevariant H202L	Escherichia coli	L-homoserine methyl ester	5.4	—	3		Open record: homoserine kinase variant H202L, L-homoserine methyl ester
homoserine kinasevariant H202L	Escherichia coli	L-homoserine propyl ester	2.7	—	1.4		Open record: homoserine kinase variant H202L, L-homoserine propyl ester
homoserine kinasevariant H205Q	Escherichia coli	ATP	0.036	0.15	0.24 calc.		Open record: homoserine kinase variant H205Q, ATP
homoserine kinasevariant H205Q	Escherichia coli	L-homoserine	0.005	—	—	pH 8	Open record: homoserine kinase variant H205Q, L-homoserine
homoserine kinasevariant R234C	Escherichia coli	ATP	0.28	0.88	0.32 calc.		Open record: homoserine kinase variant R234C, ATP
homoserine kinasevariant R234C	Escherichia coli	L-homoserine	0.022	—	—	pH 8	Open record: homoserine kinase variant R234C, L-homoserine
homoserine kinasevariant R234H	Escherichia coli	ATP	0.37	—	—		Open record: homoserine kinase variant R234H, ATP
homoserine kinasevariant R234H	Escherichia coli	ATPcosubstrate L-homoserine	—	0.13	—		Open record: homoserine kinase variant R234H, ATP
homoserine kinasevariant R234H	Escherichia coli	L-homoserine	0.073	0.13	0.56 calc.		Open record: homoserine kinase variant R234H, L-homoserine
homoserine kinasevariant R234L	Escherichia coli	ATP	0.049	—	—		Open record: homoserine kinase variant R234L, ATP
homoserine kinasevariant R234L	Escherichia coli	ATPcosubstrate L-homoserine	—	0.21	—		Open record: homoserine kinase variant R234L, ATP
homoserine kinasevariant R234L	Escherichia coli	L-homoserineTable 2	0.2	40.1	0.0050 calc.		Open record: homoserine kinase variant R234L, L-homoserine
homoserine kinasevariant R234L	Escherichia coli	L-homoserineTable 3	—	—	0.005		Open record: homoserine kinase variant R234L, L-homoserine
homoserine kinasevariant R234L	Escherichia coli	L-homoserine butyl ester	0.007	—	7 × 10⁻⁴		Open record: homoserine kinase variant R234L, L-homoserine butyl ester
homoserine kinasevariant R234L	Escherichia coli	L-homoserine ethyl ester	0.021	—	0.019		Open record: homoserine kinase variant R234L, L-homoserine ethyl ester
homoserine kinasevariant R234L	Escherichia coli	L-homoserine methyl ester	0.018	—	0.0025		Open record: homoserine kinase variant R234L, L-homoserine methyl ester
homoserine kinasevariant R234L	Escherichia coli	L-homoserine propyl ester	0.011	—	0.0075		Open record: homoserine kinase variant R234L, L-homoserine propyl ester