Values CatLog read from this paper
Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis
PMID 8999873doi:10.1074/jbc.272.3.1864Open in Browse
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CatLog read 12 records (12 values) from this paper: transketolase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| transketolasevariant D477A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 1.75 | — | 25 °C | Open record: transketolase variant D477A, D-ribose 5-phosphate |
| transketolasevariant D477A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | — | 0.595 | — | 25 °C | Open record: transketolase variant D477A, D-xylulose 5-phosphate |
| transketolasevariant D477A | Saccharomyces cerevisiae | thiamin diphosphate | — | 0.0012 | — | 25 °C | Open record: transketolase variant D477A, thiamin diphosphate |
| transketolasevariant H469A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 5.97 | — | 25 °C | Open record: transketolase variant H469A, D-ribose 5-phosphate |
| transketolasevariant H469A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | — | 0.829 | — | 25 °C | Open record: transketolase variant H469A, D-xylulose 5-phosphate |
| transketolasevariant H469A | Saccharomyces cerevisiae | thiamin diphosphate | — | 5 × 10⁻⁴ | — | 25 °C | Open record: transketolase variant H469A, thiamin diphosphate |
| transketolasevariant R359A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 5.65 | — | 25 °C | Open record: transketolase variant R359A, D-ribose 5-phosphate |
| transketolasevariant R359A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | — | 0.163 | — | 25 °C | Open record: transketolase variant R359A, D-xylulose 5-phosphate |
| transketolasevariant R359A | Saccharomyces cerevisiae | thiamin diphosphate | — | 0.0019 | — | 25 °C | Open record: transketolase variant R359A, thiamin diphosphate |
| transketolasevariant R528A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 7 | — | 25 °C | Open record: transketolase variant R528A, D-ribose 5-phosphate |
| transketolasevariant R528A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | — | 0.318 | — | 25 °C | Open record: transketolase variant R528A, D-xylulose 5-phosphate |
| transketolasevariant R528A | Saccharomyces cerevisiae | thiamin diphosphate | — | 0.0024 | — | 25 °C | Open record: transketolase variant R528A, thiamin diphosphate |