Values CatLog read from this paper
D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus. II. Mutagenic analysis of catalytically important residues
PMID 9063466doi:10.1111/j.1432-1033.1997.00213.xOpen in Browse
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CatLog read 30 records (70 values) from this paper: D-2-hydroxy-4-methylvalerate dehydrogenase from Lactobacillus delbrueckii subsp. bulgaricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-2-hydroxy-4-methylvalerate dehydrogenase | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 130 | 0.025 | 5.2 × 10³ | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenase | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 128 | 0.03 | 4.3 × 10³ | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenase | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 102 | 0.03 | 3.4 × 10³ | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenase | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 70 | 0.025 | 2.8 × 10³ | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenase | Lactobacillus delbrueckii subsp. bulgaricus | NADH | — | 0.007 | — | 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase, NADH |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant D259N | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 36 | 1.2 | 30 | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant D259N, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant D259N | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 16 | 1.7 | 9.4 | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant D259N, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant D259N | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 15 | 7.4 | 2 | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant D259N, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant D259N | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 7 | 9.65 | 0.73 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant D259N, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant E264Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 43 | 1.7 | 25 | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant E264Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant E264Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 22 | 1.6 | 14 | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant E264Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant E264Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 12 | 1.2 | 10 | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant E264Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant E264Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 5 | 1.5 | 3.3 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant E264Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H205Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 1.1 × 10³ | 0.05 | 2.2 × 10⁴ | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H205Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H205Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 590 | 0.06 | 9.8 × 10³ | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H205Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H205Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 632 | 0.14 | 4.5 × 10³ | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H205Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H205Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 330 | 0.6 | 530 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H205Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H205Q | Lactobacillus delbrueckii subsp. bulgaricus | NADH | — | 0.111 | — | 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H205Q, NADH |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.055 | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.075 | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.095 | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296Q | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.065 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296Q, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296S | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.08 | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296S, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296S | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.04 | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296S, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296S | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.06 | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296S, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant H296S | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | — | — | 0.065 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant H296S, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant R235K | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 1.2 | 1.3 | 0.92 | pH 5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant R235K, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant R235K | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 1.2 | 1.7 | 0.71 | pH 5.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant R235K, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant R235K | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 1.3 | 2.2 | 0.59 | pH 6, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant R235K, 2-oxo-4-methylvalerate |
| D-2-hydroxy-4-methylvalerate dehydrogenasevariant R235K | Lactobacillus delbrueckii subsp. bulgaricus | 2-oxo-4-methylvalerate | 1.3 | 3.4 | 0.38 | pH 6.5, 25 °C | Open record: D-2-hydroxy-4-methylvalerate dehydrogenase variant R235K, 2-oxo-4-methylvalerate |