Values CatLog read from this paper

Modified substrate specificity of L-hydroxyisocaproate dehydrogenase derived from structure-based protein engineering

PMID 9153075doi:10.1093/protein/10.3.255Open in Browse

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CatLog read 50 records (100 values) from this paper: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) from Lactobacillus confusus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)	Lactobacillus confusus	2-oxo-2-phenylacetate	1.8 × 10⁴	—	1.88 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH), 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	52	—	133	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH), 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	2-oxo-2-phenylacetate	9.5	—	32.8	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	2-oxohexanoate	2.1 × 10³	—	2.88 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	0.07	—	0.07	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	4-methyl-2-oxopentanoate	920	—	1.53 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	phenylpyruvate	6.4 × 10³	—	4.92 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236S	Lactobacillus confusus	pyruvate	20	—	1.82	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236S, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236V	Lactobacillus confusus	2-oxo-2-phenylacetate	0.2	—	0.00526	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236V, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236V	Lactobacillus confusus	2-oxohexanoate	6.9 × 10³	—	1.92 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236V, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236V	Lactobacillus confusus	4-methyl-2-oxopentanoate	1 × 10³	—	400	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236V, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236V	Lactobacillus confusus	phenylpyruvate	1.5 × 10⁴	—	1 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236V, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant F236V	Lactobacillus confusus	pyruvate	0.1	—	0.01	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant F236V, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	2-oxo-2-phenylacetate	92	—	6.13	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	2-oxohexanoate	7.6 × 10³	—	7.6 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	0.2	—	0.357	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	4-methyl-2-oxopentanoate	2.5 × 10³	—	3.91 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	phenylpyruvate	2.5 × 10³	—	2.87 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant G234V/G235D	Lactobacillus confusus	pyruvate	340	—	65.4	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant G234V/G235D, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	2-oxo-2-phenylacetate	520	—	158	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	2-oxohexanoate	5.6 × 10³	—	2.67 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	0.3	—	0.0469	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	4-methyl-2-oxopentanoate	4.4 × 10³	—	7.33 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	phenylpyruvate	3.5 × 10³	—	2.19 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239A	Lactobacillus confusus	pyruvate	0.9	—	0.012	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239A, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239F	Lactobacillus confusus	2-oxo-2-phenylacetate	20	—	6.06	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239F, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239F	Lactobacillus confusus	2-oxohexanoate	320	—	941	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239F, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239F	Lactobacillus confusus	4-methyl-2-oxopentanoate	420	—	677	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239F, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239F	Lactobacillus confusus	phenylpyruvate	350	—	1.46 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239F, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	2-oxo-2-phenylacetate	150	—	10	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	2-oxohexanoate	7.9 × 10³	—	3.76 × 10⁴	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	0.4	—	1.08	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	4-methyl-2-oxopentanoate	1.3 × 10⁴	—	2.03 × 10⁵	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	phenylpyruvate	1.1 × 10⁴	—	2.24 × 10⁵	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M	Lactobacillus confusus	pyruvate	100	—	5.88	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M/T245A	Lactobacillus confusus	2-oxo-2-phenylacetate	0.1	—	0.013	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M/T245A, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M/T245A	Lactobacillus confusus	2-oxohexanoate	6.58	—	0.411	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M/T245A, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M/T245A	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	0.075	—	0.015	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M/T245A, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M/T245A	Lactobacillus confusus	4-methyl-2-oxopentanoate	28	—	3.84	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M/T245A, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239M/T245A	Lactobacillus confusus	phenylpyruvate	25	—	3.09	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239M/T245A, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239W	Lactobacillus confusus	2-oxo-2-phenylacetate	22	—	2.97	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239W, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239W	Lactobacillus confusus	2-oxohexanoate	2.4 × 10³	—	2.42 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239W, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239W	Lactobacillus confusus	4-methyl-2-oxopentanoate	3.4 × 10³	—	2.62 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239W, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239W	Lactobacillus confusus	phenylpyruvate	4.1 × 10³	—	5.06 × 10³	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239W, phenylpyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant L239W	Lactobacillus confusus	pyruvate	0.2	—	0.00465	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant L239W, pyruvate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant T245A	Lactobacillus confusus	2-oxo-2-phenylacetate	1.3	—	0.0684	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant T245A, 2-oxo-2-phenylacetate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant T245A	Lactobacillus confusus	2-oxohexanoate	27	—	22.5	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant T245A, 2-oxohexanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant T245A	Lactobacillus confusus	3,3-dimethyl-2-oxobutanoate	32	—	16	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant T245A, 3,3-dimethyl-2-oxobutanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant T245A	Lactobacillus confusus	4-methyl-2-oxopentanoate	210	—	117	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant T245A, 4-methyl-2-oxopentanoate
L-2-hydroxyisocaproate dehydrogenase (L-HicDH)variant T245A	Lactobacillus confusus	phenylpyruvate	51	—	66.2	pH 7, 30 °C	Open record: L-2-hydroxyisocaproate dehydrogenase (L-HicDH) variant T245A, phenylpyruvate