Values CatLog read from this paper
Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase
PMID 9182530doi:10.1074/jbc.272.24.15106Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 33 records (50 values) from this paper: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Escherichia coli, Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 17 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | 1,3-diphosphoglycerate | 0.074 | 0.08 | 0.93 calc. | pH 7, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), 1,3-diphosphoglycerate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.12 | 1.1 | 0.11 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | erythrose | 0.36 | 90 | 0.0040 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), erythrose |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | erythrose 4-phosphate | 20 | 0.51 | 39 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 0.9 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | NAD+E4P dehydrogenase assay (forward); no phosphate | — | 0.8 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase) | Escherichia coli | NADH | — | 0.05 | — | pH 7, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase), NADH |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311A | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.035 | 0.41 | 0.085 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311A, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311A | Escherichia coli | erythrose 4-phosphate | 3.5 | 0.8 | 4.4 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311A, erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311A | Escherichia coli | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 1 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311A, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311A | Escherichia coli | NAD+E4P dehydrogenase assay (forward); no phosphate | — | 4 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311A, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311Y | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.02 | 0.3 | 0.067 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311Y, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311Y | Escherichia coli | erythrose 4-phosphate | 0.6 | 0.7 | 0.86 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311Y, erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311Y | Escherichia coli | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 2.2 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311Y, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant C311Y | Escherichia coli | NAD+E4P dehydrogenase assay (forward); no phosphate | — | 5 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant C311Y, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant E32D | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.1 | 1.8 | 0.056 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant E32D, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant E32D | Escherichia coli | erythrose 4-phosphate | 7.4 | 1.9 | 3.9 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant E32D, erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant H176N | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.003 | 3 | 0.0010 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant H176N, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant H176N | Escherichia coli | erythrose 4-phosphate | 0.21 | 0.24 | 0.88 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant H176N, erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant H176N | Escherichia coli | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 0.73 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant H176N, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant H176N | Escherichia coli | NAD+E4P dehydrogenase assay (forward); no phosphate | — | 0.5 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant H176N, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant K209G/L210A | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.0014 | — | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant K209G/L210A, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant M179T | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.11 | 1 | 0.11 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant M179T, D-glyceraldehyde 3-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant M179T | Escherichia coli | erythrose 4-phosphate | 4 | 0.7 | 5.7 calc. | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant M179T, erythrose 4-phosphate |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant M179T | Escherichia coli | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 1 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant M179T, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant M179T | Escherichia coli | NAD+E4P dehydrogenase assay (forward); no phosphate | — | 3.5 | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant M179T, NAD+ |
| GapB-encoded protein (erythrose-4-phosphate dehydrogenase)variant V206S/D207S/K209G/L210A | Escherichia coli | D-glyceraldehyde 3-phosphate | 0.0011 | — | — | pH 8.9, 25 °C | Open record: GapB-encoded protein (erythrose-4-phosphate dehydrogenase) variant V206S/D207S/K209G/L210A, D-glyceraldehyde 3-phosphate |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | 1,3-diphosphoglycerate | 65 | 0.005 | 1.3 × 10⁴ calc. | pH 7, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 1,3-diphosphoglycerate |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | D-glyceraldehyde 3-phosphate | 76 | 0.9 | 84 calc. | pH 8.9, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), D-glyceraldehyde 3-phosphate |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | erythrose 4-phosphate | 0.1 | 3.3 | 0.030 calc. | pH 8.9, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), erythrose 4-phosphate |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | NAD+oxidative phosphorylation of G3P (forward); 50 mM K2HPO4 | — | 0.09 | — | pH 8.9, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), NAD+ |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | NAD+Table II footnote E4PDH assay: 40 mM triethanolamine buffer | — | 0.08 | — | pH 8.9, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), NAD+ |
| glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Bacillus stearothermophilus | NADH | — | 0.011 | — | pH 7, 25 °C | Open record: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), NADH |