Values CatLog read from this paper
Chemical mechanism of the fructose-6-phosphate,2-kinase reaction from the pH dependence of kinetic parameters of site-directed mutants of active site basic residues
PMID 9220964doi:10.1021/bi970639oOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 33 records (76 values) from this paper: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.092 | 0.04 | 2.3 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity | Rattus norvegicus | beta-D-fructose 6-phosphateapproximate kcat | 0.071 | — | — | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity | Rattus norvegicus | beta-D-fructose 6-phosphate | — | — | 5.9 | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity | Rattus norvegicus | MgATP | — | 0.1 | 0.92 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity | Rattus norvegicus | MgATP | — | — | 1.2 | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172A | Rattus norvegicus | beta-D-fructose 6-phosphate | 3.9 × 10⁻⁴ | 24 | 1.6 × 10⁻⁵ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172A, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172A | Rattus norvegicus | MgATP | — | 0.44 | 9 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172A, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172E | Rattus norvegicus | MgATP | 0.0011 | 3.2 | 3.4 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172E, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172H | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.0058 | 7.2 | 8 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172H, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172H | Rattus norvegicus | beta-D-fructose 6-phosphate | — | — | 6.1 × 10⁻⁴ | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172H, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172H | Rattus norvegicus | MgATP | — | 4.5 | 0.0013 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172H, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172R | Rattus norvegicus | beta-D-fructose 6-phosphate | 3.9 × 10⁻⁴ | 26 | 1.5 × 10⁻⁵ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172R, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K172R | Rattus norvegicus | MgATP | — | 0.063 | 0.0063 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K172R, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K51A | Rattus norvegicus | MgATP | 0.001 | 1.3 | 7.7 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K51A, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K51H | Rattus norvegicus | beta-D-fructose 6-phosphate | — | — | 2.3 × 10⁻⁴ | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K51H, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant K51H | Rattus norvegicus | MgATP | 0.001 | 4.9 | 2 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant K51H, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136K | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.1 | 0.64 | 0.16 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136K, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136K | Rattus norvegicus | MgATP | — | 3.9 | 0.026 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136K, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136L | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.053 | 58 | 9.2 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136L, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136L | Rattus norvegicus | MgATP | — | 0.73 | 0.073 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136L, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136S | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.11 | 10 | 0.011 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136S, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R136S | Rattus norvegicus | MgATP | — | 1.1 | 0.1 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R136S, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R193H | Rattus norvegicus | MgATP | 0.0018 | 6.8 | 2.6 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R193H, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R193L | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.01 | 25 | 4 × 10⁻⁴ | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R193L, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R193L | Rattus norvegicus | MgATP | — | 0.43 | 0.023 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R193L, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R78H | Rattus norvegicus | MgATP | 0.01 | 2.8 | 0.0036 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R78H, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R78L | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.05 | 9 | 0.0056 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R78L, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R78L | Rattus norvegicus | MgATP | — | 2.7 | 0.019 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R78L, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R79H | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.012 | 0.17 | 0.071 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R79H, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R79H | Rattus norvegicus | beta-D-fructose 6-phosphate | — | — | 0.083 | 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R79H, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R79H | Rattus norvegicus | MgATP | — | 0.053 | 0.23 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R79H, MgATP |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R79L | Rattus norvegicus | beta-D-fructose 6-phosphate | 0.0073 | 0.8 | 0.0092 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R79L, beta-D-fructose 6-phosphate |
| fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activityvariant R79L | Rattus norvegicus | MgATP | — | 0.63 | 0.012 | pH 7.5, 30 °C | Open record: fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase, testis isozyme (RT2K), kinase activity variant R79L, MgATP |