Values CatLog read from this paper
Identification of a flexible loop region (297-313) of urokinase-type plasminogen activator, which helps determine its catalytic activity
PMID 9295329doi:10.1074/jbc.272.38.23818Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (60 values) from this paper: urokinase-type plasminogen activator (u-PA) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 0.0053 | 0.051 | 0.1045 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA), L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 3 | 0.078 | 38.5 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA), L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 4.5 × 10⁻⁴ | 0.051 | 0.0088 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 3.3 | 0.087 | 38.3 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301D | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 7.8 × 10⁻⁴ | 0.06 | 0.013 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301D, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301D | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 4.5 | 0.082 | 54.8 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301D, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301H | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 0.025 | 0.055 | 0.455 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301H, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant E301H | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 1.38 | 0.089 | 15.5 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant E301H, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 1.28 × 10⁻⁴ | 0.054 | 0.0023 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 1 | 0.077 | 13 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300H | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 0.00108 | 0.052 | 0.0208 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300H, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300H | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 5.8 | 0.075 | 77.8 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300H, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300W | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 1.42 × 10⁻⁴ | 0.059 | 0.0023 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300W, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K300W | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 0.45 | 0.074 | 6 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K300W, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K313A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 0.0055 | 0.054 | 0.1018 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K313A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant K313A | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 3.2 | 0.08 | 39.7 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant K313A, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant S303E | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 0.0053 | 0.053 | 0.1007 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant S303E, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant S303E | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 3 | 0.077 | 38.8 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant S303E, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant Y306G | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidesingle-chain activator 1.0-20.0 µM; recombinant; refolded | 4.8 × 10⁻⁴ | 0.058 | 0.0083 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant Y306G, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |
| urokinase-type plasminogen activator (u-PA)variant Y306G | Homo sapiens | L-pyroglutamyl-glycyl-L-arginine 4-nitroanilidetwo-chain activator 5.0-20.0 nM; two-chain UK | 0.37 | 0.085 | 4.3 | pH 7.4 | Open record: urokinase-type plasminogen activator (u-PA) variant Y306G, L-pyroglutamyl-glycyl-L-arginine 4-nitroanilide |