Values CatLog read from this paper
Augmented hydrolysis of diisopropyl fluorophosphate in engineered mutants of phosphotriesterase
PMID 9325279doi:10.1074/jbc.272.41.25596Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 70 records (137 values) from this paper: phosphotriesterase from Pseudomonas diminuta.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 67 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphotriesterase | Pseudomonas diminuta | diethylphenylphosphate | 0.029 | 0.33 | 0.088 calc. | Open record: phosphotriesterase, diethylphenylphosphate | |
| phosphotriesterase | Pseudomonas diminuta | diisopropyl fluorophosphate | 290 | 0.57 | 510 calc. | Open record: phosphotriesterase, diisopropyl fluorophosphate | |
| phosphotriesterase | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 1.5 | 86 | 0.017 calc. | Open record: phosphotriesterase, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterase | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 5.1 × 10³ | 0.3 | 1.7 × 10⁴ calc. | pH 9 | Open record: phosphotriesterase, paraoxon |
| phosphotriesterase | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 2.5 × 10³ | 0.67 | 3.7 × 10³ calc. | pH 9 | Open record: phosphotriesterase, paraoxon |
| phosphotriesterase | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 780 | 0.052 | 1.5 × 10⁴ calc. | pH 9 | Open record: phosphotriesterase, paraoxon |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | diethylphenylphosphate | 0.038 | 0.99 | 0.038 calc. | Open record: phosphotriesterase variant F132H, diethylphenylphosphate | |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | diisopropyl fluorophosphate | 1.4 × 10³ | 1.2 | 1.2 × 10³ calc. | Open record: phosphotriesterase variant F132H, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 1.3 | 440 | 0.0030 calc. | Open record: phosphotriesterase variant F132H, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 6.6 × 10³ | 0.35 | 1.9 × 10⁴ calc. | pH 9 | Open record: phosphotriesterase variant F132H, paraoxon |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 2.8 × 10³ | 1 | 2.8 × 10³ calc. | pH 9 | Open record: phosphotriesterase variant F132H, paraoxon |
| phosphotriesterasevariant F132H | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 2.5 × 10³ | 0.16 | 1.6 × 10⁴ calc. | pH 9 | Open record: phosphotriesterase variant F132H, paraoxon |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | diethylphenylphosphate | 0.013 | 2.4 | 0.0054 calc. | Open record: phosphotriesterase variant F132H/F306H, diethylphenylphosphate | |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | diisopropyl fluorophosphate | 3.1 × 10³ | 10 | 310 calc. | Open record: phosphotriesterase variant F132H/F306H, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 0.13 | 69 | 0.0019 calc. | Open record: phosphotriesterase variant F132H/F306H, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 2 × 10³ | 16 | 120 calc. | pH 9 | Open record: phosphotriesterase variant F132H/F306H, paraoxon |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 67 | 5 | 13 calc. | pH 9 | Open record: phosphotriesterase variant F132H/F306H, paraoxon |
| phosphotriesterasevariant F132H/F306H | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 230 | 14 | 16 calc. | pH 9 | Open record: phosphotriesterase variant F132H/F306H, paraoxon |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | diethylphenylphosphate | 0.018 | 8.2 | 0.0022 calc. | Open record: phosphotriesterase variant F132H/F306Y, diethylphenylphosphate | |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | diisopropyl fluorophosphate | 1.4 × 10³ | 3.1 | 450 calc. | Open record: phosphotriesterase variant F132H/F306Y, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 1.5 | 420 | 0.0036 calc. | Open record: phosphotriesterase variant F132H/F306Y, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 86 | 2.8 | 31 calc. | pH 9 | Open record: phosphotriesterase variant F132H/F306Y, paraoxon |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 750 | 6 | 120 calc. | pH 9 | Open record: phosphotriesterase variant F132H/F306Y, paraoxon |
| phosphotriesterasevariant F132H/F306Y | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | — | — | 0.28 | pH 9 | Open record: phosphotriesterase variant F132H/F306Y, paraoxon |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | diethylphenylphosphate | 0.047 | 1.4 | 0.034 calc. | Open record: phosphotriesterase variant F132Y, diethylphenylphosphate | |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | diisopropyl fluorophosphate | 2.3 × 10³ | 2.4 | 960 calc. | Open record: phosphotriesterase variant F132Y, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 1.3 | 120 | 0.011 calc. | Open record: phosphotriesterase variant F132Y, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 1.9 × 10³ | 0.71 | 2.7 × 10³ calc. | pH 9 | Open record: phosphotriesterase variant F132Y, paraoxon |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 740 | 1.2 | 620 calc. | pH 9 | Open record: phosphotriesterase variant F132Y, paraoxon |
| phosphotriesterasevariant F132Y | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 1.7 × 10³ | 0.3 | 5.7 × 10³ calc. | pH 9 | Open record: phosphotriesterase variant F132Y, paraoxon |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | diethylphenylphosphate | 0.0049 | 1.3 | 0.0038 calc. | Open record: phosphotriesterase variant F132Y/F306H, diethylphenylphosphate | |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | diisopropyl fluorophosphate | 2.4 × 10³ | 13 | 180 calc. | Open record: phosphotriesterase variant F132Y/F306H, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 0.14 | 77 | 0.0018 calc. | Open record: phosphotriesterase variant F132Y/F306H, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 860 | 4.4 | 200 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306H, paraoxon |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 85 | 4.4 | 19 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306H, paraoxon |
| phosphotriesterasevariant F132Y/F306H | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 140 | 3.4 | 41 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306H, paraoxon |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | diethylphenylphosphate | 0.0028 | 2.1 | 0.0013 calc. | Open record: phosphotriesterase variant F132Y/F306Y, diethylphenylphosphate | |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | diisopropyl fluorophosphate | 1.3 × 10³ | 4.8 | 270 calc. | Open record: phosphotriesterase variant F132Y/F306Y, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 1.49 | 210 | 0.0071 calc. | Open record: phosphotriesterase variant F132Y/F306Y, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 590 | 9.5 | 62 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306Y, paraoxon |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 27 | 2.9 | 9.3 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306Y, paraoxon |
| phosphotriesterasevariant F132Y/F306Y | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 230 | 3.2 | 72 calc. | pH 9 | Open record: phosphotriesterase variant F132Y/F306Y, paraoxon |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | diethylphenylphosphate | 0.0024 | 0.25 | 0.0096 calc. | Open record: phosphotriesterase variant F306E, diethylphenylphosphate | |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | diisopropyl fluorophosphate | — | — | 2.4 | Open record: phosphotriesterase variant F306E, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 0.085 | 120 | 7.1 × 10⁻⁴ calc. | Open record: phosphotriesterase variant F306E, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 38 | 4 | 9.5 calc. | pH 9 | Open record: phosphotriesterase variant F306E, paraoxon |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 1.2 | 0.34 | 3.5 calc. | pH 9 | Open record: phosphotriesterase variant F306E, paraoxon |
| phosphotriesterasevariant F306E | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 5.1 | 2.3 | 2.2 calc. | pH 9 | Open record: phosphotriesterase variant F306E, paraoxon |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | diethylphenylphosphate | 0.011 | 2.5 | 0.0044 calc. | Open record: phosphotriesterase variant F306H, diethylphenylphosphate | |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | diisopropyl fluorophosphate | 1.4 × 10³ | 16 | 88 calc. | Open record: phosphotriesterase variant F306H, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 0.19 | 53 | 0.0036 calc. | Open record: phosphotriesterase variant F306H, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 2.3 × 10³ | 2.4 | 960 calc. | pH 9 | Open record: phosphotriesterase variant F306H, paraoxon |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 370 | 4 | 92 calc. | pH 9 | Open record: phosphotriesterase variant F306H, paraoxon |
| phosphotriesterasevariant F306H | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 320 | 1.8 | 180 calc. | pH 9 | Open record: phosphotriesterase variant F306H, paraoxon |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | diethylphenylphosphate | 0.0043 | 1.3 | 0.0033 calc. | Open record: phosphotriesterase variant F306K, diethylphenylphosphate | |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | diisopropyl fluorophosphate | 120 | 51 | 2.4 calc. | Open record: phosphotriesterase variant F306K, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 0.23 | 160 | 0.0014 calc. | Open record: phosphotriesterase variant F306K, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 1.5 × 10³ | 17 | 88 calc. | pH 9 | Open record: phosphotriesterase variant F306K, paraoxon |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 31 | 2.2 | 14 calc. | pH 9 | Open record: phosphotriesterase variant F306K, paraoxon |
| phosphotriesterasevariant F306K | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 440 | 14 | 31 calc. | pH 9 | Open record: phosphotriesterase variant F306K, paraoxon |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | diethylphenylphosphate | 0.022 | 4.4 | 0.0050 calc. | Open record: phosphotriesterase variant F306Y, diethylphenylphosphate | |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | diisopropyl fluorophosphate | 990 | 1.5 | 660 calc. | Open record: phosphotriesterase variant F306Y, diisopropyl fluorophosphate | |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | O,S-dimethyl N-acetylphosphoramidothioate | 3.3 | 150 | 0.022 calc. | Open record: phosphotriesterase variant F306Y, O,S-dimethyl N-acetylphosphoramidothioate | |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 7 × 10³ | 8.6 | 810 calc. | pH 9 | Open record: phosphotriesterase variant F306Y, paraoxon |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 360 | 6.6 | 55 calc. | pH 9 | Open record: phosphotriesterase variant F306Y, paraoxon |
| phosphotriesterasevariant F306Y | Pseudomonas diminuta | paraoxonZn2+-reconstituted enzyme | 870 | 1.9 | 460 calc. | pH 9 | Open record: phosphotriesterase variant F306Y, paraoxon |
| phosphotriesterasevariant W131K | Pseudomonas diminuta | diisopropyl fluorophosphateTable II | 0.33 | 2.48 | 0.13 calc. | Open record: phosphotriesterase variant W131K, diisopropyl fluorophosphate | |
| phosphotriesterasevariant W131K | Pseudomonas diminuta | diisopropyl fluorophosphateFigure 1 | — | — | 0.13 | Open record: phosphotriesterase variant W131K, diisopropyl fluorophosphate | |
| phosphotriesterasevariant W131K | Pseudomonas diminuta | paraoxonCo2+-reconstituted enzyme | 0.85 | 0.58 | 1.5 calc. | pH 9 | Open record: phosphotriesterase variant W131K, paraoxon |
| phosphotriesterasevariant W131K | Pseudomonas diminuta | paraoxonCd2+-reconstituted enzyme | 2.1 | 0.95 | 2.2 calc. | pH 9 | Open record: phosphotriesterase variant W131K, paraoxon |