Values CatLog read from this paper

Cephalosporin substrate specificity determinants of TEM-1 beta-lactamase

PMID 9360991doi:10.1074/jbc.272.46.29144Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (78 values) from this paper: TEM-1 β-lactamase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.2.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
TEM-1 β-lactamase	Escherichia coli	ampicillin	1428	0.05	2.86 × 10⁴	pH 7, 30 °C	Open record: TEM-1 β-lactamase, ampicillin
TEM-1 β-lactamase	Escherichia coli	benzylpenicillin	1284	0.075	1.71 × 10⁴	pH 7, 30 °C	Open record: TEM-1 β-lactamase, benzylpenicillin
TEM-1 β-lactamase	Escherichia coli	cefotaxime	—	—	2.77	pH 7, 30 °C	Open record: TEM-1 β-lactamase, cefotaxime
TEM-1 β-lactamase	Escherichia coli	cephaloridine	947	0.697	1.36 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase, cephaloridine
TEM-1 β-lactamase	Escherichia coli	cephalothin	115	0.347	331	pH 7, 30 °C	Open record: TEM-1 β-lactamase, cephalothin
TEM-1 β-lactamasevariant A237T	Escherichia coli	ampicillin	302	0.063	4.83 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T, ampicillin
TEM-1 β-lactamasevariant A237T	Escherichia coli	benzylpenicillin	196	0.027	7.26 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T, benzylpenicillin
TEM-1 β-lactamasevariant A237T	Escherichia coli	cefotaxime	—	—	3.47	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T, cefotaxime
TEM-1 β-lactamasevariant A237T	Escherichia coli	cephaloridine	854	0.337	2.53 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T, cephaloridine
TEM-1 β-lactamasevariant A237T	Escherichia coli	cephalothin	76	0.077	989	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T, cephalothin
TEM-1 β-lactamasevariant A237T/E240C	Escherichia coli	ampicillin	257	0.049	5.24 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T/E240C, ampicillin
TEM-1 β-lactamasevariant A237T/E240C	Escherichia coli	benzylpenicillin	257	0.055	4.67 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T/E240C, benzylpenicillin
TEM-1 β-lactamasevariant A237T/E240C	Escherichia coli	cefotaxime	—	—	3.92	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T/E240C, cefotaxime
TEM-1 β-lactamasevariant A237T/E240C	Escherichia coli	cephaloridine	804	0.378	2.23 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T/E240C, cephaloridine
TEM-1 β-lactamasevariant A237T/E240C	Escherichia coli	cephalothin	58	0.097	598	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant A237T/E240C, cephalothin
TEM-1 β-lactamasevariant E240C	Escherichia coli	ampicillin	391	0.13	3.01 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant E240C, ampicillin
TEM-1 β-lactamasevariant E240C	Escherichia coli	benzylpenicillin	246	0.045	5.47 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant E240C, benzylpenicillin
TEM-1 β-lactamasevariant E240C	Escherichia coli	cefotaxime	—	—	2.42	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant E240C, cefotaxime
TEM-1 β-lactamasevariant E240C	Escherichia coli	cephaloridine	789	0.747	1.06 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant E240C, cephaloridine
TEM-1 β-lactamasevariant E240C	Escherichia coli	cephalothin	32	0.066	482	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant E240C, cephalothin
TEM-1 β-lactamasevariant W165S	Escherichia coli	ampicillin	448	0.063	7.11 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S, ampicillin
TEM-1 β-lactamasevariant W165S	Escherichia coli	benzylpenicillin	899	0.132	6.81 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S, benzylpenicillin
TEM-1 β-lactamasevariant W165S	Escherichia coli	cefotaxime	—	—	0.544	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S, cefotaxime
TEM-1 β-lactamasevariant W165S	Escherichia coli	cephaloridine	398	1.012	393	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S, cephaloridine
TEM-1 β-lactamasevariant W165S	Escherichia coli	cephalothin	71	0.411	173	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S, cephalothin
TEM-1 β-lactamasevariant W165S/A237T/E240C	Escherichia coli	ampicillin	138	0.048	2.88 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S/A237T/E240C, ampicillin
TEM-1 β-lactamasevariant W165S/A237T/E240C	Escherichia coli	benzylpenicillin	272	0.049	5.55 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S/A237T/E240C, benzylpenicillin
TEM-1 β-lactamasevariant W165S/A237T/E240C	Escherichia coli	cefotaxime	—	—	3.01	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S/A237T/E240C, cefotaxime
TEM-1 β-lactamasevariant W165S/A237T/E240C	Escherichia coli	cephaloridine	1.02 × 10³	0.689	1.48 × 10³	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S/A237T/E240C, cephaloridine
TEM-1 β-lactamasevariant W165S/A237T/E240C	Escherichia coli	cephalothin	50	0.103	485	pH 7, 30 °C	Open record: TEM-1 β-lactamase variant W165S/A237T/E240C, cephalothin