Values CatLog read from this paper
Identification of catalytically important residues in yeast transketolase
PMID 9398292doi:10.1021/bi971606bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (28 values) from this paper: transketolase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| transketolase | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 0.146 | — | pH 7.6, 25 °C | Open record: transketolase, D-ribose 5-phosphate |
| transketolase | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 46.3 | 0.07 | 660 calc. | pH 7.6, 25 °C | Open record: transketolase, D-xylulose 5-phosphate |
| transketolase | Saccharomyces cerevisiae | thiamin diphosphate | — | 6 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase, thiamin diphosphate |
| transketolasevariant H263A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 0.093 | — | pH 7.6, 25 °C | Open record: transketolase variant H263A, D-ribose 5-phosphate |
| transketolasevariant H263A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 0.23 | 0.023 | 10 calc. | pH 7.6, 25 °C | Open record: transketolase variant H263A, D-xylulose 5-phosphate |
| transketolasevariant H263A | Saccharomyces cerevisiae | thiamin diphosphate | — | 0.0012 | — | pH 7.6, 25 °C | Open record: transketolase variant H263A, thiamin diphosphate |
| transketolasevariant H30A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 0.29 | — | pH 7.6, 25 °C | Open record: transketolase variant H30A, D-ribose 5-phosphate |
| transketolasevariant H30A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 0.69 | 1.01 | 0.68 calc. | pH 7.6, 25 °C | Open record: transketolase variant H30A, D-xylulose 5-phosphate |
| transketolasevariant H30A | Saccharomyces cerevisiae | thiamin diphosphate | — | 8 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase variant H30A, thiamin diphosphate |
| transketolasevariant H30N | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 0.09 | — | — | pH 7.6, 25 °C | Open record: transketolase variant H30N, D-xylulose 5-phosphate |
| transketolasevariant H30N | Saccharomyces cerevisiae | thiamin diphosphate | — | 5 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase variant H30N, thiamin diphosphate |
| transketolasevariant H481A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 0.15 | — | pH 7.6, 25 °C | Open record: transketolase variant H481A, D-ribose 5-phosphate |
| transketolasevariant H481A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 2.04 | 1.24 | 1.65 calc. | pH 7.6, 25 °C | Open record: transketolase variant H481A, D-xylulose 5-phosphate |
| transketolasevariant H481A | Saccharomyces cerevisiae | thiamin diphosphate | — | 3 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase variant H481A, thiamin diphosphate |
| transketolasevariant H481Q | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 0.88 | 4.08 | 0.22 calc. | pH 7.6, 25 °C | Open record: transketolase variant H481Q, D-xylulose 5-phosphate |
| transketolasevariant H481Q | Saccharomyces cerevisiae | thiamin diphosphate | — | 7 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase variant H481Q, thiamin diphosphate |
| transketolasevariant H481S | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 2.54 | 3.35 | 0.758 calc. | pH 7.6, 25 °C | Open record: transketolase variant H481S, D-xylulose 5-phosphate |
| transketolasevariant H481S | Saccharomyces cerevisiae | thiamin diphosphate | — | 5 × 10⁻⁴ | — | pH 7.6, 25 °C | Open record: transketolase variant H481S, thiamin diphosphate |
| transketolasevariant H69A | Saccharomyces cerevisiae | D-ribose 5-phosphate | — | 0.193 | — | pH 7.6, 25 °C | Open record: transketolase variant H69A, D-ribose 5-phosphate |
| transketolasevariant H69A | Saccharomyces cerevisiae | D-xylulose 5-phosphate | 0.69 | 0.4 | 1.7 calc. | pH 7.6, 25 °C | Open record: transketolase variant H69A, D-xylulose 5-phosphate |
| transketolasevariant H69A | Saccharomyces cerevisiae | thiamin diphosphate | — | 0.0018 | — | pH 7.6, 25 °C | Open record: transketolase variant H69A, thiamin diphosphate |