Values CatLog read from this paper
Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily: a site-directed mutagenesis study with the Pseudomonas sp. strain CBS3 4-chlorobenzoate:coenzyme A ligase
PMID 9398293doi:10.1021/bi971262pOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (30 values) from this paper: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) | Pseudomonas sp. | 4-chlorobenzoate | 34 | 0.014 | 2.4 × 10³ calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase), 4-chlorobenzoate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) | Pseudomonas sp. | adenosine 5'-triphosphate | 54 | 0.308 | 180 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase), adenosine 5'-triphosphate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) | Pseudomonas sp. | coenzyme A | 39 | 0.085 | 460 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase), coenzyme A |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant E306Q | Pseudomonas sp. | 4-chlorobenzoate | 0.7 | 0.253 | 2.8 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant E306Q, 4-chlorobenzoate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant E306Q | Pseudomonas sp. | adenosine 5'-triphosphate | 0.9 | 0.321 | 2.8 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant E306Q, adenosine 5'-triphosphate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant E306Q | Pseudomonas sp. | coenzyme A | 0.8 | 0.168 | 4.8 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant E306Q, coenzyme A |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G163I | Pseudomonas sp. | 4-chlorobenzoate | 0.036 | 0.105 | 0.34 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G163I, 4-chlorobenzoate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G163I | Pseudomonas sp. | adenosine 5'-triphosphate | 0.039 | 1.314 | 0.030 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G163I, adenosine 5'-triphosphate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G163I | Pseudomonas sp. | coenzyme A | 0.04 | 0.062 | 0.65 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G163I, coenzyme A |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G166I | Pseudomonas sp. | 4-chlorobenzoate | 2.3 | 0.073 | 32 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G166I, 4-chlorobenzoate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G166I | Pseudomonas sp. | adenosine 5'-triphosphate | 3.3 | 0.981 | 3.4 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G166I, adenosine 5'-triphosphate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant G166I | Pseudomonas sp. | coenzyme A | 2.7 | 0.108 | 25 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant G166I, coenzyme A |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant K169M | Pseudomonas sp. | 4-chlorobenzoate | 9.8 | 0.02 | 490 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant K169M, 4-chlorobenzoate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant K169M | Pseudomonas sp. | adenosine 5'-triphosphate | 16 | 0.83 | 19 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant K169M, adenosine 5'-triphosphate |
| 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase)variant K169M | Pseudomonas sp. | coenzyme A | 9.4 | 0.076 | 120 calc. | pH 7.5, 25 °C | Open record: 4-chlorobenzoate:coenzyme A ligase (4-CBA:CoA ligase) variant K169M, coenzyme A |