Values CatLog read from this paper
Heparinase I from Flavobacterium heparinum. Role of positive charge in enzymatic activity
PMID 9417072doi:10.1074/jbc.273.1.248Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (24 values) from this paper: heparinase I from Flavobacterium heparinum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| heparinase I | Flavobacterium heparinum | heparin | 92 | 0.0102 | 9.0 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I, heparin |
| heparinase Ivariant K132A | Flavobacterium heparinum | heparin | 12 | 0.0081 | 1.5 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I variant K132A, heparin |
| heparinase Ivariant K198A | Flavobacterium heparinum | heparin | 35 | 0.008 | 4.4 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I variant K198A, heparin |
| heparinase Ivariant K198A/K199A | Flavobacterium heparinum | heparin | 4.7 | 0.0054 | 870 calc. | pH 7, 30 °C | Open record: heparinase I variant K198A/K199A, heparin |
| heparinase Ivariant K198D/K199D | Flavobacterium heparinum | heparin | 4.9 | 0.0042 | 1.2 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I variant K198D/K199D, heparin |
| heparinase Ivariant K198R/K199R | Flavobacterium heparinum | heparin | 90 | 0.0084 | 1.1 × 10⁴ calc. | pH 7, 30 °C | Open record: heparinase I variant K198R/K199R, heparin |
| heparinase Ivariant K199A | Flavobacterium heparinum | heparin | 6 | 0.0076 | 790 calc. | pH 7, 30 °C | Open record: heparinase I variant K199A, heparin |
| heparinase Ivariant K208A/K209A | Flavobacterium heparinum | heparin | 68 | 0.0096 | 7.1 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I variant K208A/K209A, heparin |
| heparinase Ivariant K208D/K209D | Flavobacterium heparinum | heparin | 90 | 0.0092 | 9.8 × 10³ calc. | pH 7, 30 °C | Open record: heparinase I variant K208D/K209D, heparin |
| heparinase Ivariant K208R/K209R | Flavobacterium heparinum | heparin | 94 | 0.0091 | 1.0 × 10⁴ calc. | pH 7, 30 °C | Open record: heparinase I variant K208R/K209R, heparin |
| heparinase Ivariant N200A | Flavobacterium heparinum | heparin | 91 | 0.0089 | 1.0 × 10⁴ calc. | pH 7, 30 °C | Open record: heparinase I variant N200A, heparin |
| heparinase Ivariant R141A | Flavobacterium heparinum | heparin | 82 | 0.0078 | 1.1 × 10⁴ calc. | pH 7, 30 °C | Open record: heparinase I variant R141A, heparin |