Values CatLog read from this paper
Substrate specificities and kinetic properties of proteinase A from the yeast Saccharomyces cerevisiae and the development of a novel substrate
PMID 9644256doi:10.1093/oxfordjournals.jbchem.a022072Open in Browse
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CatLog read 41 records (123 values) from this paper: proteinase A, pepsin, renin from Saccharomyces cerevisiae, Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pepsin | Sus scrofa | N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide | 21.1 | 0.00617 | 3.42 × 10³ | 30 °C | Open record: pepsin, N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide |
| pepsin | Sus scrofa | Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA | 20.7 | 0.00581 | 3.56 × 10³ | pH 3, 30 °C | Open record: pepsin, Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA |
| pepsin | Sus scrofa | Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA | 9.11 | 0.00834 | 1.09 × 10³ | pH 3, 30 °C | Open record: pepsin, Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA |
| pepsin | Sus scrofa | Suc-Leu-Phe-Ala-Leu-Glu-Val-Ala-Tyr-Asp-MCA | 2 | 0.0076 | 263 | pH 3, 30 °C | Open record: pepsin, Suc-Leu-Phe-Ala-Leu-Glu-Val-Ala-Tyr-Asp-MCA |
| proteinase A | Saccharomyces cerevisiae | glycyl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 19.7 | 0.019 | 1.04 × 10³ | pH 4.5, 30 °C | Open record: proteinase A, glycyl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-alanyl-L-leucyl-L-seryl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 10.9 | 0.01 | 1.09 × 10³ | pH 4.5, 30 °C | Open record: proteinase A, L-alanyl-L-leucyl-L-seryl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 14.4 | 0.012 | 1.2 × 10³ | pH 4.5, 30 °C | Open record: proteinase A, L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-alpha-aspartyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 18.6 | 0.026 | 715 | pH 4.5, 30 °C | Open record: proteinase A, L-alpha-aspartyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 14.7 | 0.02 | 735 | pH 4.5, 30 °C | Open record: proteinase A, L-arginyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-leucyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 13.8 | 0.014 | 986 | pH 4.5, 30 °C | Open record: proteinase A, L-leucyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-alanyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 3.5 | 0.037 | 95 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-alanyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-alpha-aspartyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 5.8 | 0.033 | 176 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-alpha-aspartyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-arginyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.7 | 0.094 | 71 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-arginyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-leucyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.8 | 0.031 | 219 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-leucyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 15.9 | 0.02 | 795 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-alanyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 1.7 | 0.017 | 100 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-arginyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 9.4 | 0.097 | 97 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-arginyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-leucyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 8.4 | 0.017 | 494 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-leucyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alanyl-L-leucine | 1.5 | 0.042 | 36 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alanyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alpha-aspartyl-L-leucine | 2 | 0.068 | 29 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-alpha-aspartyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-alanine | 6.7 | 0.095 | 71 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-alanine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-arginine | 2.7 | 0.126 | 21 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-arginine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-aspartic acid | 1.7 | 0.024 | 71 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-aspartic acid |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 4 | 0.03 | 133 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-serine | 4.7 | 0.25 | 19 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-serine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-leucyl-L-leucine | 1.7 | 0.017 | 100 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-leucyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-seryl-L-leucine | 0.9 | 0.073 | 12 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-seryl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alanyl-L-seryl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.7 | 0.015 | 447 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alanyl-L-seryl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-alpha-aspartyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 1.9 | 0.028 | 68 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-alpha-aspartyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-arginyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 3.3 | 0.093 | 35 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-arginyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-leucyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 10.6 | 0.038 | 279 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-leucyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-prolyl-L-seryl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 10.4 | 0.028 | 371 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-prolyl-L-seryl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-lysyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 2.4 | 0.037 | 65 | pH 4.5, 30 °C | Open record: proteinase A, L-lysyl-L-seryl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | L-seryl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine | 6.6 | 0.015 | 440 | pH 4.5, 30 °C | Open record: proteinase A, L-seryl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-4-nitro-L-phenylalanyl-L-arginyl-L-leucine |
| proteinase A | Saccharomyces cerevisiae | N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide | 18.1 | 0.00618 | 2.93 × 10³ | pH 4.5, 30 °C | Open record: proteinase A, N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide |
| proteinase A | Saccharomyces cerevisiae | Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA | 18.5 | 0.00607 | 3.04 × 10³ | pH 4.5, 30 °C | Open record: proteinase A, Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA |
| proteinase A | Saccharomyces cerevisiae | Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA | 8.25 | 0.0393 | 210 | pH 4.5, 30 °C | Open record: proteinase A, Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA |
| proteinase A | Saccharomyces cerevisiae | Suc-Leu-Phe-Ala-Leu-Glu-Val-Ala-Tyr-Asp-MCA | 3.73 | 0.0181 | 206 | pH 4.5, 30 °C | Open record: proteinase A, Suc-Leu-Phe-Ala-Leu-Glu-Val-Ala-Tyr-Asp-MCA |
| renin | Sus scrofa | N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide | 0.8 | 0.56 | 1.4 | 30 °C | Open record: renin, N-[(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-alanyl-L-prolyl-L-alanyl-L-lysyl-L-phenylalanyl-L-phenylalanyl-L-arginyl-L-leucyl-N6-(2,4-dinitrophenyl)-L-lysinamide |
| renin | Sus scrofa | Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA | 0.9 | 0.37 | 2.4 | pH 6.8, 30 °C | Open record: renin, Suc-Ala-Pro-Ala-Lys-Phe-Phe-Arg-Leu-MCA |
| renin | Sus scrofa | Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA | 10.2 | 0.00253 | 4.03 × 10³ | pH 6.8, 30 °C | Open record: renin, Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-MCA |