Values CatLog read from this paper
Directed mutations in the poorly defined region of porcine liver fructose-1,6-bisphosphatase significantly affect catalysis and the mechanism of AMP inhibition
PMID 9651342doi:10.1074/jbc.273.28.17511Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (18 values) from this paper: fructose-1,6-bisphosphatase from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose-1,6-bisphosphatase | Sus scrofa | D-fructose 1,6-bisphosphate | 21 | 0.0028 | 7.5 × 10³ calc. | pH 7.5, 25 °C | Open record: fructose-1,6-bisphosphatase, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatase | Sus scrofa | D-fructose 1,6-bisphosphate | 9.1 | 0.0022 | 4.1 × 10³ calc. | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant D68E | Sus scrofa | D-fructose 1,6-bisphosphate | 6.4 | 0.00131 | 4.9 × 10³ calc. | pH 7.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant D68E, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant D74A | Sus scrofa | D-fructose 1,6-bisphosphate | 4 × 10⁻⁴ | — | — | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant D74A, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant D74E | Sus scrofa | D-fructose 1,6-bisphosphate | 1.2 | 0.00103 | 1.2 × 10³ calc. | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant D74E, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant D74N | Sus scrofa | D-fructose 1,6-bisphosphate | 0.01 | — | — | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant D74N, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant K71A | Sus scrofa | D-fructose 1,6-bisphosphate | 16 | 0.0028 | 5.7 × 10³ calc. | pH 7.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant K71A, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant K71M/K72M | Sus scrofa | D-fructose 1,6-bisphosphate | 34 | 0.0016 | 2.1 × 10⁴ calc. | pH 7.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant K71M/K72M, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant N64A | Sus scrofa | D-fructose 1,6-bisphosphate | 7.1 | 0.00142 | 5.0 × 10³ calc. | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant N64A, D-fructose 1,6-bisphosphate |
| fructose-1,6-bisphosphatasevariant N64Q | Sus scrofa | D-fructose 1,6-bisphosphate | 5.4 | 0.0017 | 3.2 × 10³ calc. | pH 8.5, 25 °C | Open record: fructose-1,6-bisphosphatase variant N64Q, D-fructose 1,6-bisphosphate |