Values CatLog read from this paper
Acylamino acid-releasing enzyme from the thermophilic archaeon Pyrococcus horikoshii
PMID 9651372doi:10.1074/jbc.273.28.17726Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (24 values) from this paper: acylamino acid-releasing enzyme (AAREP), acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanine p-nitroanilide | 17.3 | 18.4 | 0.940 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-alanine p-nitroanilide |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanyl-alanine | 790 | 7.6 | 100 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-alanyl-alanine |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanyl-alanyl-alanyl-alanine | 281 | 13 | 22 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-alanyl-alanyl-alanyl-alanine |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | 330 | 11 | 30 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 0.689 | — | pH 5.4, 60 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 3.6 | — | pH 5.4, 75 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 3.85 | — | pH 5.4, 80 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 18 | — | pH 5.4, 90 °C | Open record: acylamino acid-releasing enzyme (AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanine p-nitroanilide | 144 | 20.2 | 7.13 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-alanine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanyl-alanine | 9.9 × 10³ | 8.6 | 1.2 × 10³ calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-alanyl-alanine |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-alanyl-alanyl-alanyl-alanine | 2.09 × 10³ | 15.2 | 138 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-alanyl-alanyl-alanyl-alanine |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | 3.2 × 10³ | 12.9 | 250 calc. | pH 5.4, 85 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 0.876 | — | pH 5.4, 60 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 4.12 | — | pH 5.4, 75 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 3.99 | — | pH 5.4, 80 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-leucine p-nitroanilide |
| acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP) | Pyrococcus horikoshii | Nα-acetyl-leucine p-nitroanilide | — | 19.7 | — | pH 5.4, 90 °C | Open record: acylamino acid-releasing enzyme (HAAREP, heat-activated AAREP), Nα-acetyl-leucine p-nitroanilide |