Values CatLog read from this paper
Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues
PMID 9665712doi:10.1021/bi980409pOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (43 values) from this paper: methylglyoxal synthase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylglyoxal synthase | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 220 | 0.2 | 1.1 × 10³ | pH 7 | Open record: methylglyoxal synthase, dihydroxyacetone phosphate |
| methylglyoxal synthase | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor); apparent kcat | 203 | — | — | pH 7 | Open record: methylglyoxal synthase, dihydroxyacetone phosphate |
| methylglyoxal synthase | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi) | 324 | 0.16 | 2.0 × 10³ calc. | pH 7 | Open record: methylglyoxal synthase, dihydroxyacetone phosphate |
| methylglyoxal synthase | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 600 | — | — | pH 7 | Open record: methylglyoxal synthase, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.0648 | 0.33 | 0.2 | pH 7 | Open record: methylglyoxal synthase variant D101E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101E | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.0514 | 2.81 | 0.0183 calc. | pH 7 | Open record: methylglyoxal synthase variant D101E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi) | 5.4 | — | — | pH 7 | Open record: methylglyoxal synthase variant D101E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101N | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.0476 | 0.65 | 0.073 | pH 7 | Open record: methylglyoxal synthase variant D101N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101N | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.0227 | 0.98 | 0.023 calc. | pH 7 | Open record: methylglyoxal synthase variant D101N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D101N | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi) | 0.047 | 0.64 | 0.073 calc. | pH 7 | Open record: methylglyoxal synthase variant D101N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D20E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.959 | 0.18 | 5.3 | pH 7 | Open record: methylglyoxal synthase variant D20E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D20E | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.83 | — | — | pH 7 | Open record: methylglyoxal synthase variant D20E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.248 | 0.38 | 0.65 | pH 7 | Open record: methylglyoxal synthase variant D71E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71E | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.225 | 0.73 | 0.31 calc. | pH 7 | Open record: methylglyoxal synthase variant D71E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi) | 0.83 | — | — | pH 7 | Open record: methylglyoxal synthase variant D71E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71N | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.0918 | 0.12 | 0.77 | pH 7 | Open record: methylglyoxal synthase variant D71N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71N | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.0706 | 0.13 | 0.54 calc. | pH 7 | Open record: methylglyoxal synthase variant D71N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D71N | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi) | 0.083 | 0.08 | 1.0 calc. | pH 7 | Open record: methylglyoxal synthase variant D71N, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D91E | Escherichia coli | dihydroxyacetone phosphateno phosphate (0.0 mM Pi); Michaelis-Menten fit (eq 1) | 0.564 | 0.41 | 1.4 | pH 7 | Open record: methylglyoxal synthase variant D91E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D91E | Escherichia coli | dihydroxyacetone phosphatewith 0.3 mM Pi (allosteric inhibitor) | 0.148 | — | — | pH 7 | Open record: methylglyoxal synthase variant D91E, dihydroxyacetone phosphate |
| methylglyoxal synthasevariant D91N | Escherichia coli | dihydroxyacetone phosphate | 1.6 | 0.56 | 2.9 calc. | pH 7 | Open record: methylglyoxal synthase variant D91N, dihydroxyacetone phosphate |