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Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants

PMID 9726873doi:10.1128/aem.64.9.3290-3299.1998Open in Browse

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CatLog read 59 records (177 values) from this paper: muconate cycloisomerase from Pseudomonas putida, Acinetobacter calcoaceticus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 5.5.1.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
muconate cycloisomerase	Acinetobacter calcoaceticus	2-chloro-cis,cis-muconate	0.28	0.29	1	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 2-chloro-cis,cis-muconate
muconate cycloisomerase	Acinetobacter calcoaceticus	2-methyl-cis,cis-muconate	78	0.49	162	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 2-methyl-cis,cis-muconate
muconate cycloisomerase	Acinetobacter calcoaceticus	3-fluoro-cis,cis-muconate	43	0.1	400	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 3-fluoro-cis,cis-muconate
muconate cycloisomerase	Acinetobacter calcoaceticus	3-methyl-cis,cis-muconate	30	0.099	300	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 3-methyl-cis,cis-muconate
muconate cycloisomerase	Acinetobacter calcoaceticus	cis,cis-muconate	62	0.13	480	pH 7.5, 25 °C	Open record: muconate cycloisomerase, cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.055	0.026	2.2	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	2-chloro-cis,cis-muconate	0.82	0.059	13.8	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 2-chloro-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	2-methyl-cis,cis-muconate	65	0.04	1.62 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 2-methyl-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	3-chloro-cis,cis-muconate	1.07	0.32	3.3	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 3-chloro-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	3-fluoro-cis,cis-muconate	148	0.097	1.53 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 3-fluoro-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	3-methyl-cis,cis-muconate	9.8	0.006	1.65 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase, 3-methyl-cis,cis-muconate
muconate cycloisomerase	Pseudomonas putida	cis,cis-muconate	210	0.04	5.2 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase, cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.048	0.044	1.12	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	2-chloro-cis,cis-muconate	0.25	0.06	4.2	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	2-methyl-cis,cis-muconate	107	0.059	1.8 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	3-chloro-cis,cis-muconate	16	0.18	90	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	3-fluoro-cis,cis-muconate	100	0.1	1 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	3-methyl-cis,cis-muconate	11.3	0.0097	1.18 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant A271S	Pseudomonas putida	cis,cis-muconate	118	0.035	3.3 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant A271S, cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.53	0.078	6.8	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	2-chloro-cis,cis-muconate	0.8	0.037	22	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	2-methyl-cis,cis-muconate	167	0.068	2.5 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	3-chloro-cis,cis-muconate	7.7	0.69	11.2	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	3-fluoro-cis,cis-muconate	133	0.15	880	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	3-methyl-cis,cis-muconate	4.5	0.016	280	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant F329I	Pseudomonas putida	cis,cis-muconate	43	0.047	900	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant F329I, cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.12	0.093	1.3	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	2-chloro-cis,cis-muconate	0.58	0.047	12.5	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	2-methyl-cis,cis-muconate	10.8	0.058	180	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	3-chloro-cis,cis-muconate	3.3	0.045	73	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	3-fluoro-cis,cis-muconate	20	0.074	270	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	3-methyl-cis,cis-muconate	12.3	0.0072	1.7 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant I54V	Pseudomonas putida	cis,cis-muconate	42	0.096	430	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V, cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.37	0.045	8.2	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	2-chloro-cis,cis-muconate	0.17	0.033	5.3	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	2-methyl-cis,cis-muconate	23	0.057	400	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	3-chloro-cis,cis-muconate	1.8	0.21	9	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	3-fluoro-cis,cis-muconate	82	0.1	780	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	3-methyl-cis,cis-muconate	5.2	0.0064	820	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant I54V/F329I	Pseudomonas putida	cis,cis-muconate	9	0.14	63	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant I54V/F329I, cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.0102	0.04	0.25	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	2-chloro-cis,cis-muconate	0.053	0.034	1.58	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	2-methyl-cis,cis-muconate	16.7	0.18	97	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	3-chloro-cis,cis-muconate	6.3	0.55	11.5	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	3-fluoro-cis,cis-muconate	87	0.23	370	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	3-methyl-cis,cis-muconate	1.58	0.012	137	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant K276N	Pseudomonas putida	cis,cis-muconate	35	0.074	480	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant K276N, cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	2,4-dichloro-cis,cis-muconate	0.0147	0.039	0.37	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 2,4-dichloro-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	2-chloro-cis,cis-muconate	0.0122	0.036	0.33	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	2-methyl-cis,cis-muconate	5.3	0.11	47	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	3-chloro-cis,cis-muconate	0.87	0.076	11.3	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 3-chloro-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	3-fluoro-cis,cis-muconate	68	0.063	1.08 × 10³	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	3-methyl-cis,cis-muconate	0.9	0.0091	100	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant L333V	Pseudomonas putida	cis,cis-muconate	1.13	0.052	22	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant L333V, cis,cis-muconate
muconate cycloisomerasevariant Y59W	Pseudomonas putida	2-chloro-cis,cis-muconate	0.25	0.017	14.5	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant Y59W, 2-chloro-cis,cis-muconate
muconate cycloisomerasevariant Y59W	Pseudomonas putida	2-methyl-cis,cis-muconate	62	0.069	880	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant Y59W, 2-methyl-cis,cis-muconate
muconate cycloisomerasevariant Y59W	Pseudomonas putida	3-fluoro-cis,cis-muconate	53	0.15	350	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant Y59W, 3-fluoro-cis,cis-muconate
muconate cycloisomerasevariant Y59W	Pseudomonas putida	3-methyl-cis,cis-muconate	2.3	0.015	162	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant Y59W, 3-methyl-cis,cis-muconate
muconate cycloisomerasevariant Y59W	Pseudomonas putida	cis,cis-muconate	18	0.047	400	pH 7.5, 25 °C	Open record: muconate cycloisomerase variant Y59W, cis,cis-muconate