Values CatLog read from this paper
Kinetic analysis of an inhibitor-resistant variant of the OHIO-1 beta-lactamase, an SHV-family class A enzyme
PMID 9735103doi:10.1042/bj3330395Open in Browse
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CatLog read 12 records (32 values) from this paper: OHIO-1 beta-lactamase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| OHIO-1 beta-lactamase | — | 7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid | 50 | 0.075 | 600 | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, 7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid |
| OHIO-1 beta-lactamase | — | Benzylpenicillin | 345 | 0.017 | 2 × 10⁴ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, Benzylpenicillin |
| OHIO-1 beta-lactamase | — | Carbenicillin | 320 | 0.017 | 1.9 × 10⁴ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, Carbenicillin |
| OHIO-1 beta-lactamase | — | Cefaloridine | 1023 | 0.096 | 1 × 10⁴ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, Cefaloridine |
| OHIO-1 beta-lactamase | — | Nitrocefin | 115 | 0.011 | 9 × 10³ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, Nitrocefin |
| OHIO-1 beta-lactamase | — | Sulbactam | 0.4 | — | — | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase, Sulbactam |
| OHIO-1 beta-lactamasevariant M69I | — | 7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid | 9 | 0.006 | 1.5 × 10³ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, 7-(thienyl-2-acetamido)-3-[2-(4-N,N-dimethylaminophenylazo)pyridinium-methyl]-3-cephem-4-carboxylic acid |
| OHIO-1 beta-lactamasevariant M69I | — | Benzylpenicillin | 234 | 0.006 | 3.9 × 10⁴ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, Benzylpenicillin |
| OHIO-1 beta-lactamasevariant M69I | — | Carbenicillin | 131 | 0.003 | 4.4 × 10⁴ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, Carbenicillin |
| OHIO-1 beta-lactamasevariant M69I | — | Cefaloridine | 235 | 0.038 | 6 × 10³ | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, Cefaloridine |
| OHIO-1 beta-lactamasevariant M69I | — | Nitrocefin | 13 | 0.022 | 600 | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, Nitrocefin |
| OHIO-1 beta-lactamasevariant M69I | — | Sulbactam | 2 | — | — | pH 7.4, 25 °C | Open record: OHIO-1 beta-lactamase variant M69I, Sulbactam |