Values CatLog read from this paper
Activating a zymogen without proteolytic processing: mutation of Lys15 and Asn194 activates trypsinogen
PMID 9819212doi:10.1021/bi980951dOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (50 values) from this paper: anionic trypsinogen II, anionic trypsin II from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| anionic trypsin IIvariant D194N | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 1.9 | 0.146 | 13 | pH 8, 25 °C | Open record: anionic trypsin II variant D194N, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant H40F | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 38 | 0.0016 | 2.5 × 10⁴ | pH 8, 25 °C | Open record: anionic trypsin II variant H40F, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant H40F | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 49 | 0.015 | 3.2 × 10³ | pH 8, 25 °C | Open record: anionic trypsin II variant H40F, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant I16G | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | — | — | 0.114 | pH 8, 25 °C | Open record: anionic trypsin II variant I16G, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant ΔI16 | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | — | — | 0.0019 | pH 8, 25 °C | Open record: anionic trypsin II variant ΔI16, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant ΔI16 | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | — | — | 0.0013 | pH 8, 25 °C | Open record: anionic trypsin II variant ΔI16, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant ΔI16V17 | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 0.0011 | 1.4 | 8 × 10⁻⁴ | pH 8, 25 °C | Open record: anionic trypsin II variant ΔI16V17, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsin IIvariant ΔI16V17 | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | — | — | 6.7 × 10⁻⁴ | pH 8, 25 °C | Open record: anionic trypsin II variant ΔI16V17, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant I16G | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | — | — | 6.9 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant I16G, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant I16G/D194N | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 5.7 × 10⁻⁵ | 0.3 | 1.9 × 10⁻⁴ | pH 8, 25 °C | Open record: anionic trypsinogen II variant I16G/D194N, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant I16G/D194N | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 1.3 × 10⁻⁵ | 0.3 | 4.4 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant I16G/D194N, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant K15A | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 5 × 10⁻⁴ | 1.2 | 0.0044 | pH 8, 25 °C | Open record: anionic trypsinogen II variant K15A, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant K15A | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 0.0013 | 4 | 3.4 × 10⁻⁴ | pH 8, 25 °C | Open record: anionic trypsinogen II variant K15A, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17 | Rattus norvegicus | carbobenzoxy-Arg thiobenzyl ester | — | — | 0.0016 | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17, carbobenzoxy-Arg thiobenzyl ester |
| anionic trypsinogen IIvariant ΔI16V17 | Rattus norvegicus | carbobenzoxy-Lys thiobenzyl ester | — | — | 0.0016 | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17, carbobenzoxy-Lys thiobenzyl ester |
| anionic trypsinogen IIvariant ΔI16V17 | Rattus norvegicus | D-Val-Leu-Lys thiobenzyl ester | — | — | 0.0072 | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17, D-Val-Leu-Lys thiobenzyl ester |
| anionic trypsinogen IIvariant ΔI16V17 | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 2.8 × 10⁻⁵ | 1.2 | 2.5 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17 | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 3 × 10⁻⁶ | 0.16 | 1.9 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17/D194N | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 7.1 × 10⁻⁵ | 0.018 | 0.0039 | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17/D194N, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17/D194N | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 1.2 × 10⁻⁵ | 0.051 | 2.4 × 10⁻⁴ | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17/D194N, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17/H40F | Rattus norvegicus | Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin | 8 × 10⁻⁵ | 0.9 | 8.4 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17/H40F, Tos-Gly-Pro-Arg-7-amino-4-methylcoumarin |
| anionic trypsinogen IIvariant ΔI16V17/H40F | Rattus norvegicus | Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin | 8 × 10⁻⁶ | 0.27 | 3.1 × 10⁻⁵ | pH 8, 25 °C | Open record: anionic trypsinogen II variant ΔI16V17/H40F, Tos-Gly-Pro-Lys-7-amino-4-methylcoumarin |