Values CatLog read from this paper
Conserved residues in the mechanism of the E. coli Class II FBP-aldolase
PMID 9878448doi:10.1006/jmbi.1998.2376Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (36 values) from this paper: fructose 1,6-bisphosphate aldolase (Class II) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 18 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose 1,6-bisphosphate aldolase (Class II)variant D109A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.0028 | 0.18 | 0.016 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D109A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D109A | Escherichia coli | dihydroxyacetone phosphate | 0.33 | 1.5 | 0.22 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D109A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D144A | Escherichia coli | D-fructose 1,6-bisphosphate | 2 | 0.94 | 2.1 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D144A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D144A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.33 | 0.17 | 2.0 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D144A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D144A | Escherichia coli | dihydroxyacetone phosphate | 0.67 | 0.03 | 22 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D144A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D288A | Escherichia coli | D-fructose 1,6-bisphosphate | 2.17 | 1 | 2.2 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D288A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D288A | Escherichia coli | D-fructose 1,6-bisphosphate | 1.83 | 0.88 | 2.1 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D288A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D288A | Escherichia coli | dihydroxyacetone phosphate | 1.5 | 0.03 | 50 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D288A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D290A | Escherichia coli | D-fructose 1,6-bisphosphate | 7 | 0.77 | 9.1 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D290A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D290A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.83 | 0.24 | 3.5 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D290A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D290A | Escherichia coli | dihydroxyacetone phosphate | 5 | 0.32 | 16 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D290A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D329A | Escherichia coli | D-fructose 1,6-bisphosphate | 12.3 | 0.92 | 13 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D329A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D329A | Escherichia coli | D-fructose 1,6-bisphosphate | 5.17 | 0.9 | 5.7 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D329A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant D329A | Escherichia coli | dihydroxyacetone phosphate | 6.17 | 0.05 | 120 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant D329A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N286A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.19 | 1.07 | 0.18 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N286A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N286A | Escherichia coli | D-fructose 1,6-bisphosphate | 0.067 | 0.23 | 0.29 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N286A, D-fructose 1,6-bisphosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N286A | Escherichia coli | dihydroxyacetone phosphate | 0.238 | 0.43 | 0.55 calc. | pH 8, 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N286A, dihydroxyacetone phosphate |
| fructose 1,6-bisphosphate aldolase (Class II)variant N286D | Escherichia coli | D-fructose 1,6-bisphosphate | 0.001 | 0.37 | 0.0027 calc. | 30 °C | Open record: fructose 1,6-bisphosphate aldolase (Class II) variant N286D, D-fructose 1,6-bisphosphate |