Values CatLog read from this paper

Functional analysis of conserved cysteines in heparan sulfate N-deacetylase-N-sulfotransferases

PMID 9890952doi:10.1074/jbc.274.4.1966Open in Browse

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CatLog read 18 records (54 values) from this paper: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.8.2.8

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)Rattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)4.20.162637 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST), chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)Rattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.080.144137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST), N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C159ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)1.440.0433337 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C159A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C159ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.20.282137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C159A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)4.20.1213437 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.30.242137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486FRattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.020.0970.237 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486F, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486GRattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.060.1470.437 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486G, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486VRattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.230.18137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486V, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C56ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)2.90.083637 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C56A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C56ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.10.164137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C56A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C586ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)0.260.035737 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C586A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C601ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)0.290.0291037 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C601A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C601ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.050.048137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C601A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C751ARattus norvegicuschemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)4.020.1422837 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C751A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C751ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.10.107137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C751A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C818ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.120.174137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C818A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)
heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C828ARattus norvegicusN-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)0.080.124137 °COpen record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C828A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride)