Values CatLog read from this paper
Functional analysis of conserved cysteines in heparan sulfate N-deacetylase-N-sulfotransferases
PMID 9890952doi:10.1074/jbc.274.4.1966Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (54 values) from this paper: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 4.2 | 0.16 | 26 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST), chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.08 | 0.144 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST), N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C159A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 1.44 | 0.043 | 33 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C159A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C159A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.2 | 0.282 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C159A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 4.2 | 0.121 | 34 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.3 | 0.242 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486F | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.02 | 0.097 | 0.2 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486F, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486G | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.06 | 0.147 | 0.4 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486G, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C486V | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.23 | 0.18 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C486V, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C56A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 2.9 | 0.08 | 36 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C56A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C56A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.1 | 0.164 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C56A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C586A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 0.26 | 0.035 | 7 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C586A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C601A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 0.29 | 0.029 | 10 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C601A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C601A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.05 | 0.048 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C601A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C751A | Rattus norvegicus | chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) | 4.02 | 0.142 | 28 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C751A, chemically N-deacetylated Escherichia coli K5 polysaccharide (repeating glucuronyl-glucosamine disaccharides) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C751A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.1 | 0.107 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C751A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C818A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.12 | 0.174 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C818A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |
| heparan sulfate N-deacetylase-N-sulfotransferase (NDANST)variant C828A | Rattus norvegicus | N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) | 0.08 | 0.124 | 1 | 37 °C | Open record: heparan sulfate N-deacetylase-N-sulfotransferase (NDANST) variant C828A, N-[3H]acetyl-labeled Escherichia coli K5 polysaccharide (repeating glucuronyl-N-acetylglucosamine disaccharides, chemically N-deacetylated and re-N-acetylated with [3H]acetic anhydride) |