CatLogenzyme kinetics catalog

EC 1.1.1.45: L-gulonate 3-dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 51 records for EC 1.1.1.45 from 2 papers, for 3 organisms. Names in these papers: L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY), L-gulonate 3-dehydrogenase / lambda-crystallin.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 12 records for EC 1.1.1.45 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.1.45 in Browse.

Papers

PaperOrganismsRecords
Structural and functional characterization of rabbit and human L-gulonate 3-dehydrogenaseHomo sapiens, Oryctolagus cuniculus41
Dimeric crystal structure of rabbit L-gulonate 3-dehydrogenase/lambda-crystallin: insights into the catalytic mechanismOryctolagus cuniculus (rabbit)10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensAcetoacetate—9.9—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-3-hydroxybutyrate—2—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-gulonateNADH absorbance at 340 nm; at saturating coenzyme—0.22—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-gulonatewith 5 mM Pi—1—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-threonate—2.1—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNAD+—0.01—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNADH—8 × 10⁻⁴—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNADP+—0.52—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculus2,3-diketogulonate—6.1—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusAcetoacetatepurified from rabbit liver—8.3—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusAcetoacetate—9.9—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusacetoacetate—9.7—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyratedehydrogenase direction; NADH absorbance at 340 nm; Table 2—2.3—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyratedehydrogenase direction; NADH absorbance at 340 nm; Table 2—2.2—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyrateMichaelis constants from secondary plots; in the text—2—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonatepurified from rabbit liver—0.21—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonate—0.18—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonate—1.5—pH 8.5, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-threonatepurified from rabbit liver—2.9—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-threonate—1.2—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+dehydrogenase direction; NADH absorbance at 340 nm; Table 2—0.011—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+dehydrogenase direction; NADH absorbance at 340 nm; Table 2—0.01—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+Michaelis constants from secondary plots; cosubstrate HBA—0.009—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHreductase direction; NADH absorbance at 340 nm; apparent Km—5 × 10⁻⁴—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHreductase direction; NADH absorbance at 340 nm; apparent Km—6 × 10⁻⁴—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHMichaelis constants from secondary plots; in the text—5 × 10⁻⁴—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADP+purified from rabbit liver—0.89—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADP+—0.67—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—14—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusL-gulonateNADP+-linked L-gulonate oxidation; cosubstrate NADP+—18.5—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusNAD+—1.3—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusNADP+—0.2—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusacetoacetate—18—25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—0.49—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonateNADP+-linked L-gulonate oxidation; cosubstrate NADP+—18.5—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonatedehydrogenase direction; with 5 mM Pi; cosubstrate NAD+—2.4—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusNAD+—0.069—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusNADP+—0.64—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—4.4—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusL-gulonatedehydrogenase direction; with 5 mM Pi—29—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusNAD+—0.033—pH 7, 25 °CPMID 15809331Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant D36R/Q41NOryctolagus cuniculus (rabbit)L-gulonateNAD+-linked activity; cosubstrate NAD+—3.3—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant D36R/Q41NOryctolagus cuniculus (rabbit)L-gulonateNADP+-linked activity; cosubstrate NADP+—2.6—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant D36R/Q41NOryctolagus cuniculus (rabbit)nicotinamide adenine dinucleotide (oxidized)—2.6—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant D36R/Q41NOryctolagus cuniculus (rabbit)nicotinamide adenine dinucleotide phosphate (oxidized)—0.12—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant E97QOryctolagus cuniculus (rabbit)L-gulonate—1.6—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant E97QOryctolagus cuniculus (rabbit)nicotinamide adenine dinucleotide (oxidized)—0.054—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant Q41NOryctolagus cuniculus (rabbit)L-gulonateNAD+-linked activity; cosubstrate NAD+—1.3—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant Q41NOryctolagus cuniculus (rabbit)L-gulonateNADP+-linked activity; cosubstrate NADP+—14—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant Q41NOryctolagus cuniculus (rabbit)nicotinamide adenine dinucleotide (oxidized)—0.028—pH 7, 25 °CPMID 20620150Open
L-gulonate 3-dehydrogenase / lambda-crystallinvariant Q41NOryctolagus cuniculus (rabbit)nicotinamide adenine dinucleotide phosphate (oxidized)—0.86—pH 7, 25 °CPMID 20620150Open