CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural and functional characterization of rabbit and human L-gulonate 3-dehydrogenase

PMID 15809331doi:10.1093/jb/mvi033Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 41 records (41 values) from this paper: L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY) from Oryctolagus cuniculus, Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.45

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensAcetoacetate—9.9—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-3-hydroxybutyrate—2—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-gulonateNADH absorbance at 340 nm; at saturating coenzyme—0.22—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-gulonatewith 5 mM Pi—1—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensL-threonate—2.1—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNAD+—0.01—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNADH—8 × 10⁻⁴—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Homo sapiensNADP+—0.52—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculus2,3-diketogulonate—6.1—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusAcetoacetatepurified from rabbit liver—8.3—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusAcetoacetate—9.9—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusacetoacetate—9.7—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyratedehydrogenase direction; NADH absorbance at 340 nm; Table 2—2.3—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyratedehydrogenase direction; NADH absorbance at 340 nm; Table 2—2.2—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-3-hydroxybutyrateMichaelis constants from secondary plots; in the text—2—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonatepurified from rabbit liver—0.21—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonate—0.18—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-gulonate—1.5—pH 8.5, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-threonatepurified from rabbit liver—2.9—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusL-threonate—1.2—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+dehydrogenase direction; NADH absorbance at 340 nm; Table 2—0.011—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+dehydrogenase direction; NADH absorbance at 340 nm; Table 2—0.01—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNAD+Michaelis constants from secondary plots; cosubstrate HBA—0.009—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHreductase direction; NADH absorbance at 340 nm; apparent Km—5 × 10⁻⁴—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHreductase direction; NADH absorbance at 340 nm; apparent Km—6 × 10⁻⁴—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADHMichaelis constants from secondary plots; in the text—5 × 10⁻⁴—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADP+purified from rabbit liver—0.89—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)Oryctolagus cuniculusNADP+—0.67—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—14—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusL-gulonateNADP+-linked L-gulonate oxidation; cosubstrate NADP+—18.5—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusNAD+—1.3—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant D36ROryctolagus cuniculusNADP+—0.2—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusacetoacetate—18—25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—0.49—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonateNADP+-linked L-gulonate oxidation; cosubstrate NADP+—18.5—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusL-gulonatedehydrogenase direction; with 5 mM Pi; cosubstrate NAD+—2.4—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusNAD+—0.069—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant E157QOryctolagus cuniculusNADP+—0.64—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusL-gulonateNAD+-linked L-gulonate oxidation; NADH absorbance at 340 nm—4.4—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusL-gulonatedehydrogenase direction; with 5 mM Pi—29—pH 7, 25 °COpen
L-gulonate 3-dehydrogenase (GDH; lambda-crystallin, λ-CRY)variant S124AOryctolagus cuniculusNAD+—0.033—pH 7, 25 °COpen