CatLogenzyme kinetics catalog

EC 1.1.1.82: malate dehydrogenase (NADP+)

OxidoreductasesOpen in Browse

CatLog read 19 records for EC 1.1.1.82 from 2 papers, for 3 organisms. Names in these papers: chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH), NADP-malate dehydrogenase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 75 records for EC 1.1.1.82 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.1.82 in Browse.

Papers

PaperOrganismsRecords
The catalytic site of chloroplastic NADP-dependent malate dehydrogenase contains a His/Asp pairSorghum bicolor13
Structural, immunological and kinetic comparisons of NADP-dependent malate dehydrogenases from spinach (C3) and corn (C4) chloroplastsSpinacia oleracea (spinach), Zea mays (corn)6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicolormalate2331219.41pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicolorNADPH—0.04463.4 × 10⁴pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm1.52 × 10³0.043.8 × 10⁴pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH2125——pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicolormalate0.550.1pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicolorNADPH—0.02335.6 × 10³pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm130343pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetate400——pH 6.5, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH125——pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicolormalate0.910.20.088pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicolorNADPH—0.01149.6 × 10³pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm1101.861pH 7.9, 30 °CPMID 8665917Open
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH90——pH 7.9, 30 °CPMID 8665917Open
NADP-malate dehydrogenaseSpinacia oleracea (spinach)malate6.7180.37 calc.pH 8PMID 3948869Open
NADP-malate dehydrogenaseSpinacia oleracea (spinach)NADP+623.0 calc.pH 8PMID 3948869Open
NADP-malate dehydrogenaseZea mays (corn)malate2.9750.039 calc.pH 8PMID 3948869Open
NADP-malate dehydrogenaseZea mays (corn)NADP+2.91.262.3 calc.pH 8PMID 3948869Open
NADP-malate dehydrogenaseZea mays (corn)NADPH9550.1954.90 × 10³ calc.pH 8PMID 3948869Open
NADP-malate dehydrogenaseZea mays (corn)oxaloacetate9550.2144.46 × 10³ calc.pH 8PMID 3948869Open