CatLogenzyme kinetics catalog

Values CatLog read from this paper

The catalytic site of chloroplastic NADP-dependent malate dehydrogenase contains a His/Asp pair

PMID 8665917doi:10.1111/j.1432-1033.1996.00947.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 13 records (28 values) from this paper: chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH) from Sorghum bicolor.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.82

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicolormalate2331219.41pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicolorNADPH—0.04463.4 × 10⁴pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm1.52 × 10³0.043.8 × 10⁴pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)Sorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH2125——pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicolormalate0.550.1pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicolorNADPH—0.02335.6 × 10³pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm130343pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetate400——pH 6.5, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201ASorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH125——pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicolormalate0.910.20.088pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicolorNADPH—0.01149.6 × 10³pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicoloroxaloacetate100 mM Tris/HCl; NADPH oxidation followed at 340 nm1101.861pH 7.9, 30 °COpen
chloroplastic NADP-dependent malate dehydrogenase (NADP-MDH)variant D201NSorghum bicoloroxaloacetatepH-rate profile assay: 250 mM buffer; 200 µM NADPH90——pH 7.9, 30 °COpen