EC 1.1.3.38: vanillyl-alcohol oxidase
OxidoreductasesOpen in Browse
CatLog read 95 records for EC 1.1.3.38 from 5 papers, for 3 organisms. Names in these papers: vanillyl-alcohol oxidase, eugenol oxidase (EUGO), vanillyl-alcohol oxidase (VAO).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 65 records for EC 1.1.3.38 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.3.38 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Laboratory-evolved vanillyl-alcohol oxidase produces natural vanillin | Penicillium simplicissimum | 76 |
| Discovery of a eugenol oxidase from Rhodococcus sp. strain RHA1 | Rhodococcus sp. | 6 |
| Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase | Penicillium simplicissimum | 6 |
| Dehydrogenase versus oxidase function: the interplay between substrate binding and flavin microenvironment | Rhodococcus jostii | 4 |
| Inversion of stereospecificity of vanillyl-alcohol oxidase | Penicillium simplicissimum | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| eugenol oxidase (EUGO) | Rhodococcus jostii | vanillyl alcoholelectron acceptor molecular oxygen; molecular oxygen | 9.68 | 0.2044 | 47.4 | pH 7.5, 25 °C | PMID 39781101 | Open record: eugenol oxidase (EUGO), vanillyl alcohol |
| eugenol oxidase (EUGO) | Rhodococcus jostii | vanillyl alcoholelectron acceptor 2,6-dichlorophenolindophenol (100 μM) | 0.48 | 0.0176 | 27.2 | pH 7.5, 25 °C | PMID 39781101 | Open record: eugenol oxidase (EUGO), vanillyl alcohol |
| eugenol oxidase (EUGO)variant L152P | Rhodococcus jostii | vanillyl alcoholelectron acceptor molecular oxygen; molecular oxygen | 0.02 | 0.054 | 0.37 | pH 7.5, 25 °C | PMID 39781101 | Open record: eugenol oxidase (EUGO) variant L152P, vanillyl alcohol |
| eugenol oxidase (EUGO)variant L152P | Rhodococcus jostii | vanillyl alcoholelectron acceptor 2,6-dichlorophenolindophenol (100 μM) | 0.61 | 0.868 | 0.7 | pH 7.5, 25 °C | PMID 39781101 | Open record: eugenol oxidase (EUGO) variant L152P, vanillyl alcohol |
| eugenol oxidase (EUGO) | Rhodococcus sp. | 4-(Methoxymethyl)phenol | 0.004 | 0.0023 | 2 | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), 4-(Methoxymethyl)phenol |
| eugenol oxidase (EUGO) | Rhodococcus sp. | 4-ethyl-2-methoxyphenol | 0.026 | 0.0021 | 12 | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), 4-ethyl-2-methoxyphenol |
| eugenol oxidase (EUGO) | Rhodococcus sp. | 4-hydroxy-3-methoxybenzyl alcohol | 12 | 0.04 | 300 | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), 4-hydroxy-3-methoxybenzyl alcohol |
| eugenol oxidase (EUGO) | Rhodococcus sp. | 4-hydroxy-3-methoxybenzylamine | 0.26 | 0.076 | 3.4 | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), 4-hydroxy-3-methoxybenzylamine |
| eugenol oxidase (EUGO) | Rhodococcus sp. | 5-Indanol | 2.4 | 0.023 | 100 | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), 5-Indanol |
| eugenol oxidase (EUGO) | Rhodococcus sp. | Eugenol | 3.1 | 0.001 | 3.1 × 10³ | pH 7.5, 25 °C | PMID 17419730 | Open record: eugenol oxidase (EUGO), Eugenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-amino-p-cresol | 0.017 | 0.123 | 0.14 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-amino-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-amino-p-cresol | 0.001 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-amino-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.02 | 0.02 | 1.0 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.005 | 0.002 | 2.5 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.013 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.003 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methyl-p-cresol | 0.031 | 0.021 | 1.5 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methyl-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methyl-p-cresol | 0.002 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 2-methyl-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.1 | 0.058 | 53 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.9 | 0.11 | 35 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 14 | 0.002 | 7.0 × 10³ calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 39 | 0.019 | 2.1 × 10³ calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 1.6 | 0.075 | 21 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 2.4 | 0.189 | 13 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-methylphenol | 0.005 | 0.031 | 0.16 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase, 4-methylphenol |
| vanillyl-alcohol oxidasevariant A429V | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.018 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant A429V, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant A429V | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.015 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant A429V, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant D170A/T457E | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 0.02 | 0.065 | 0.31 calc. | pH 7.5, 25 °C | PMID 10920192 | Open record: vanillyl-alcohol oxidase variant D170A/T457E, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant D170S/T457E | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 0.05 | 0.238 | 0.21 calc. | pH 7.5, 25 °C | PMID 10920192 | Open record: vanillyl-alcohol oxidase variant D170S/T457E, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-amino-p-cresol | 0.099 | 0.2 | 0.49 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-amino-p-cresol | 0.076 | 0.043 | 1.8 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.1 | 0.027 | 3.7 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.09 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.016 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.02 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methyl-p-cresol | 0.019 | 0.007 | 2.7 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methyl-p-cresol | 0.028 | 0.001 | 28 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 1.1 | 0.064 | 17 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 1.2 | 0.004 | 300 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.17 | 0.005 | 34 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.1 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.22 | 0.051 | 4.3 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.3 | 0.01 | 30 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant E502G, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-amino-p-cresol | 0.082 | 0.167 | 0.49 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-amino-p-cresol | 0.047 | 0.064 | 0.73 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.14 | 0.013 | 11 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.1 | 0.002 | 50 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.017 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.013 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methyl-p-cresol | 0.032 | 0.006 | 5.3 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methyl-p-cresol | 0.01 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.1 | 0.054 | 39 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.4 | 0.023 | 150 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.5 | 0.003 | 170 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.23 | 8 × 10⁻⁴ | 290 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.25 | 0.007 | 36 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.71 | 0.015 | 47 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F454Y, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F93Y/P189S/I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.019 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant F93Y/P189S/I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-amino-p-cresol | 0.079 | 0.118 | 0.67 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-amino-p-cresol | 0.086 | 0.05 | 1.7 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.17 | 0.041 | 4.1 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.07 | 0.001 | 70 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.022 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.02 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methyl-p-cresol | 0.063 | 0.017 | 3.7 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methyl-p-cresol | 0.02 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.1 | 0.051 | 41 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3 | 0.02 | 150 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.8 | 0.008 | 100 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.6 | 2 × 10⁻⁴ | 3.0 × 10³ calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.39 | 0.007 | 56 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 1.4 | 0.022 | 64 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant I238T/M437L | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.015 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T/M437L, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T/M437L | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.021 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant I238T/M437L, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T457E | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 1.3 | 0.168 | 7.7 calc. | pH 7.5, 25 °C | PMID 10920192 | Open record: vanillyl-alcohol oxidase variant T457E, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-amino-p-cresol | 0.087 | 0.254 | 0.34 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-amino-p-cresol | 0.046 | 0.029 | 1.6 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.12 | 0.031 | 3.9 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.1 | 0.001 | 100 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.01 | — | — | pH 8, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.017 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methyl-p-cresol | 0.025 | 0.007 | 3.6 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methyl-p-cresol | 0.023 | — | — | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.3 | 0.033 | 70 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.6 | 0.014 | 260 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.65 | 8 × 10⁻⁴ | 810 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.55 | 0.002 | 280 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.21 | 0.006 | 35 calc. | pH 7.5, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.67 | 0.01 | 67 calc. | pH 10, 25 °C | PMID 15169773 | Open record: vanillyl-alcohol oxidase variant T505S, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidase (VAO) | Penicillium simplicissimum | 4-allylphenol | 7.4 | 0.0065 | 1138 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO), 4-allylphenol |
| vanillyl-alcohol oxidase (VAO)variant Y108F | Penicillium simplicissimum | 4-allylphenol | 3.4 | 0.067 | 51 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO) variant Y108F, 4-allylphenol |
| vanillyl-alcohol oxidase (VAO)variant Y108F | Penicillium simplicissimum | vanillyl alcohol | 0.042 | 1.75 | 0.024 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO) variant Y108F, vanillyl alcohol |
| vanillyl-alcohol oxidase (VAO)variant Y108F/Y503F | Penicillium simplicissimum | 4-allylphenol | 0.13 | 0.074 | 1.8 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO) variant Y108F/Y503F, 4-allylphenol |
| vanillyl-alcohol oxidase (VAO)variant Y503F | Penicillium simplicissimum | 4-allylphenol | 3.3 | 0.021 | 157 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO) variant Y503F, 4-allylphenol |
| vanillyl-alcohol oxidase (VAO)variant Y503F | Penicillium simplicissimum | vanillyl alcohol | 0.055 | 0.99 | 0.056 | pH 7.5, 25 °C | PMID 28717004 | Open record: vanillyl-alcohol oxidase (VAO) variant Y503F, vanillyl alcohol |