Values CatLog read from this paper
Laboratory-evolved vanillyl-alcohol oxidase produces natural vanillin
PMID 15169773doi:10.1074/jbc.m312968200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 76 records (130 values) from this paper: vanillyl-alcohol oxidase from Penicillium simplicissimum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 54 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-amino-p-cresol | 0.017 | 0.123 | 0.14 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 2-amino-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-amino-p-cresol | 0.001 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 2-amino-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.02 | 0.02 | 1.0 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.005 | 0.002 | 2.5 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.013 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.003 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methyl-p-cresol | 0.031 | 0.021 | 1.5 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methyl-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 2-methyl-p-cresol | 0.002 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 2-methyl-p-cresol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.1 | 0.058 | 53 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.9 | 0.11 | 35 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 14 | 0.002 | 7.0 × 10³ calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 39 | 0.019 | 2.1 × 10³ calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 1.6 | 0.075 | 21 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 2.4 | 0.189 | 13 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidase | Penicillium simplicissimum | 4-methylphenol | 0.005 | 0.031 | 0.16 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase, 4-methylphenol |
| vanillyl-alcohol oxidasevariant A429V | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.018 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant A429V, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant A429V | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.015 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant A429V, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-amino-p-cresol | 0.099 | 0.2 | 0.49 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-amino-p-cresol | 0.076 | 0.043 | 1.8 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.1 | 0.027 | 3.7 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.09 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.016 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.02 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methyl-p-cresol | 0.019 | 0.007 | 2.7 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 2-methyl-p-cresol | 0.028 | 0.001 | 28 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 1.1 | 0.064 | 17 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 1.2 | 0.004 | 300 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.17 | 0.005 | 34 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.1 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.22 | 0.051 | 4.3 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant E502G | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.3 | 0.01 | 30 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant E502G, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-amino-p-cresol | 0.082 | 0.167 | 0.49 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-amino-p-cresol | 0.047 | 0.064 | 0.73 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.14 | 0.013 | 11 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.1 | 0.002 | 50 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.017 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.013 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methyl-p-cresol | 0.032 | 0.006 | 5.3 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 2-methyl-p-cresol | 0.01 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.1 | 0.054 | 39 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.4 | 0.023 | 150 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.5 | 0.003 | 170 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.23 | 8 × 10⁻⁴ | 290 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.25 | 0.007 | 36 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F454Y | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.71 | 0.015 | 47 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F454Y, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant F93Y/P189S/I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.019 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant F93Y/P189S/I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-amino-p-cresol | 0.079 | 0.118 | 0.67 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-amino-p-cresol | 0.086 | 0.05 | 1.7 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.17 | 0.041 | 4.1 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.07 | 0.001 | 70 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.022 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.02 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methyl-p-cresol | 0.063 | 0.017 | 3.7 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 2-methyl-p-cresol | 0.02 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.1 | 0.051 | 41 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3 | 0.02 | 150 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.8 | 0.008 | 100 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.6 | 2 × 10⁻⁴ | 3.0 × 10³ calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.39 | 0.007 | 56 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant I238T | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 1.4 | 0.022 | 64 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant I238T/M437L | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.015 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T/M437L, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant I238T/M437L | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.021 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant I238T/M437L, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-amino-p-cresol | 0.087 | 0.254 | 0.34 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-amino-p-cresol | 0.046 | 0.029 | 1.6 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-amino-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.12 | 0.031 | 3.9 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol50 mM glycine/potassium hydroxide (pH 10); air-saturated | 0.1 | 0.001 | 100 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenol | 0.01 | — | — | pH 8, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methoxy-4-methylphenolE. coli cell extract; 750 µM creosol (saturating) | 0.017 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methoxy-4-methylphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methyl-p-cresol | 0.025 | 0.007 | 3.6 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 2-methyl-p-cresol | 0.023 | — | — | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 2-methyl-p-cresol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 2.3 | 0.033 | 70 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-(methoxymethyl)phenol | 3.6 | 0.014 | 260 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-(methoxymethyl)phenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.65 | 8 × 10⁻⁴ | 810 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-allyl-2-methoxyphenol | 0.55 | 0.002 | 280 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-allyl-2-methoxyphenol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.21 | 0.006 | 35 calc. | pH 7.5, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-hydroxy-3-methoxybenzyl alcohol |
| vanillyl-alcohol oxidasevariant T505S | Penicillium simplicissimum | 4-hydroxy-3-methoxybenzyl alcohol | 0.67 | 0.01 | 67 calc. | pH 10, 25 °C | Open record: vanillyl-alcohol oxidase variant T505S, 4-hydroxy-3-methoxybenzyl alcohol |