EC 1.1.99.8: alcohol dehydrogenase (acceptor)
OxidoreductasesOpen in Browse
CatLog read 26 records for EC 1.1.99.8 from 1 paper, for 1 organism. Names in these papers: methanol dehydrogenase (MDH).
EC 1.1.99.8 is no longer current; BRENDA: “transferred to EC 1.1.2.7 and EC 1.1.2.8”.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Effects of multiple ligand binding on kinetic isotope effects in PQQ-dependent methanol dehydrogenase | Methylophilus methylotrophus | 26 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator); Michaelis-Menten fit | 1.51 | 1.2 | 1.3 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator); Michaelis-Menten fit | 2.53 | 1.2 | 2.1 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol150 mM ammonium sulfate (activator); Michaelis-Menten fit | 1.97 | 1 | 2.0 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol | 4.91 | 0.93 | 5.3 calc. | pH 9, 30 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 7.7 | 1.1 | 7.0 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 17.44 | 1.1 | 16 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol150 mM ammonium sulfate (activator) | 18.65 | 1.4 | 13 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 0.68 | — | — | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 1.49 | — | — | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 2.1 | — | — | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 1.7 | — | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol2 mM ammonium sulfate (activator) | 3 | — | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 4.4 | — | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 6.83 | — | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 3.5 | — | — | pH 9, 41 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 7.22 | — | — | pH 9, 41 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 14.5 | — | — | pH 9, 41 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanoldye-linked assay with 1 mM PES (DCPIP-coupled) | — | 1 | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanoldye-linked assay with 100 µM PES (DCPIP-coupled) | — | 0.15 | — | pH 9, 25 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d4 mM ammonium sulfate (activator) | 0.36 | 1.2 | 0.30 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d20 mM ammonium sulfate (activator) | 1.11 | 1.4 | 0.79 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d150 mM ammonium sulfate (activator) | 1.12 | 1.5 | 0.75 calc. | pH 9, 5 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d | 0.99 | 1.1 | 0.90 calc. | pH 9, 30 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d4 mM ammonium sulfate (activator) | 1.23 | 1.1 | 1.1 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d20 mM ammonium sulfate (activator) | 7.41 | 1.1 | 6.7 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d150 mM ammonium sulfate (activator) | 8.01 | 1.5 | 5.3 calc. | pH 9, 40 °C | PMID 12667088 | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |