Values CatLog read from this paper
Effects of multiple ligand binding on kinetic isotope effects in PQQ-dependent methanol dehydrogenase
PMID 12667088doi:10.1021/bi027282vOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 26 records (40 values) from this paper: methanol dehydrogenase (MDH) from Methylophilus methylotrophus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator); Michaelis-Menten fit | 1.51 | 1.2 | 1.3 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator); Michaelis-Menten fit | 2.53 | 1.2 | 2.1 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol150 mM ammonium sulfate (activator); Michaelis-Menten fit | 1.97 | 1 | 2.0 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol | 4.91 | 0.93 | 5.3 calc. | pH 9, 30 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 7.7 | 1.1 | 7.0 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 17.44 | 1.1 | 16 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol150 mM ammonium sulfate (activator) | 18.65 | 1.4 | 13 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 0.68 | — | — | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 1.49 | — | — | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 2.1 | — | — | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 1.7 | — | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol2 mM ammonium sulfate (activator) | 3 | — | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 4.4 | — | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 6.83 | — | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol1 mM ammonium sulfate (activator) | 3.5 | — | — | pH 9, 41 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol4 mM ammonium sulfate (activator) | 7.22 | — | — | pH 9, 41 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanol20 mM ammonium sulfate (activator) | 14.5 | — | — | pH 9, 41 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanoldye-linked assay with 1 mM PES (DCPIP-coupled) | — | 1 | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methanoldye-linked assay with 100 µM PES (DCPIP-coupled) | — | 0.15 | — | pH 9, 25 °C | Open record: methanol dehydrogenase (MDH), methanol |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d4 mM ammonium sulfate (activator) | 0.36 | 1.2 | 0.30 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d20 mM ammonium sulfate (activator) | 1.11 | 1.4 | 0.79 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d150 mM ammonium sulfate (activator) | 1.12 | 1.5 | 0.75 calc. | pH 9, 5 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d | 0.99 | 1.1 | 0.90 calc. | pH 9, 30 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d4 mM ammonium sulfate (activator) | 1.23 | 1.1 | 1.1 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d20 mM ammonium sulfate (activator) | 7.41 | 1.1 | 6.7 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |
| methanol dehydrogenase (MDH) | Methylophilus methylotrophus | methyl-d3 alcohol-d150 mM ammonium sulfate (activator) | 8.01 | 1.5 | 5.3 calc. | pH 9, 40 °C | Open record: methanol dehydrogenase (MDH), methyl-d3 alcohol-d |