EC 1.13.11.48: 3-hydroxy-2-methylquinolin-4-one 2,4-dioxygenase
OxidoreductasesOpen in Browse
CatLog read 42 records for EC 1.13.11.48 from 3 papers, for 1 organism. Names in these papers: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase, 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD), (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 5 records for EC 1.13.11.48 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.13.11.48 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Dioxygenases without requirement for cofactors: identification of amino acid residues involved in substrate binding and catalysis, and testing for rate-limiting steps in the reaction of… | Arthrobacter nitroguajacolicus | 30 |
| Structural basis for cofactor-independent dioxygenation of N-heteroaromatic compounds at the alpha/beta-hydrolase fold | Arthrobacter nitroguajacolicus | 6 |
| Origin of the proton-transfer step in the cofactor-free (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase: effect of the basicity of an active site His residue | Arthrobacter nitroguajacolicus | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S (WT HOD) | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 16.3 | 0.0032 | 5.1 × 10³ | pH 6.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S (WT HOD), (1H)-3-hydroxy-4-oxoquinaldine |
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S (WT HOD) | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 29.5 | 0.0048 | 6.1 × 10³ | pH 10.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S (WT HOD), (1H)-3-hydroxy-4-oxoquinaldine |
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S/D126A | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 0.19 | 0.0209 | 9 | pH 6.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S/D126A, (1H)-3-hydroxy-4-oxoquinaldine |
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S/D126A | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 20.5 | 0.0301 | 681 | pH 10.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S/D126A, (1H)-3-hydroxy-4-oxoquinaldine |
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S/H251A | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 6 × 10⁻⁴ | 0.0589 | 0.01 | pH 6.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S/H251A, (1H)-3-hydroxy-4-oxoquinaldine |
| (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant C69S/H251A | Arthrobacter nitroguajacolicus | (1H)-3-hydroxy-4-oxoquinaldine | 0.097 | 0.0868 | 1.12 | pH 10.5, 20 °C | PMID 24482238 | Open record: (1H)-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant C69S/H251A, (1H)-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 38.4 | 0.0027 | 1.4 × 10⁴ calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD), 1-H-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant D126A | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 1.05 | 0.0272 | 38.6 calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant D126A, 1-H-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant H102L | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 0.027 | 0.0233 | 1.2 calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant H102L, 1-H-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant H251A | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 0.0034 | 0.059 | 0.058 calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant H251A, 1-H-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant H38A | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 3 | 0.1595 | 19 calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant H38A, 1-H-3-hydroxy-4-oxoquinaldine |
| 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD)variant S101A | Arthrobacter nitroguajacolicus | 1-H-3-hydroxy-4-oxoquinaldine | 46.4 | 0.162 | 286 calc. | pH 8 | PMID 20080731 | Open record: 1-H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) variant S101A, 1-H-3-hydroxy-4-oxoquinaldine |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 22.9 | 0.0092 | 2.5 × 10³ calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 5.2 | 0.135 | 39 calc. | pH 5.7, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 24 | — | — | pH 8.2, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 29 | 0.266 | 110 calc. | pH 9.3, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinolinein H2O | — | — | 173 | pH 8.2, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | — | — | 187 | 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 19 | 0.058 | 330 calc. | pH 11, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 113.7 | 1.233 | 92.21 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 0.44 | 0.045 | 9.8 calc. | pH 5.1, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 8.8 | 0.48 | 18 calc. | pH 9.1, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygenin H2O | — | — | 18.3 | pH 9.1, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | — | — | 19.6 | 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/E224A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 28.2 | 0.0154 | 1.83 × 10³ calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/E224A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/E224A | Arthrobacter nitroguajacolicus | dioxygen | 79.1 | 0.711 | 111 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/E224A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/F219Y | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 22.8 | 0.0095 | 2.4 × 10³ calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/F219Y, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/F219Y | Arthrobacter nitroguajacolicus | dioxygen | 110.9 | 1.27 | 87.3 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/F219Y, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H102Q | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 10 | 0.0257 | 390 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H102Q, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H102Q | Arthrobacter nitroguajacolicus | dioxygen | 14.8 | 0.325 | 45.5 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H102Q, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H251A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.01 | 0.0055 | 1.8 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H251A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H251A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.53 | 0.298 | 1.8 calc. | pH 11, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H251A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S101A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 2.3 | 0.1907 | 12 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S101A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S101A | Arthrobacter nitroguajacolicus | dioxygen | 38.8 | 1.382 | 28.1 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S101A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S220N | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 12.2 | 0.0154 | 792 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S220N, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S220N | Arthrobacter nitroguajacolicus | dioxygen | 18.3 | 0.357 | 51.3 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S220N, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 1.3 | 0.0995 | 13 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196A | Arthrobacter nitroguajacolicus | dioxygen | 6.4 | 1.646 | 3.9 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196K | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.7 | 0.0399 | 18 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196K, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196K | Arthrobacter nitroguajacolicus | dioxygen | 3.4 | 1.6 | 2.1 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196K, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196R | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 1 | 0.0836 | 12 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196R, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196R | Arthrobacter nitroguajacolicus | dioxygen | 3.4 | 1.37 | 2.5 calc. | pH 8, 30 °C | PMID 16187153 | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196R, dioxygen |