Values CatLog read from this paper
Dioxygenases without requirement for cofactors: identification of amino acid residues involved in substrate binding and catalysis, and testing for rate-limiting steps in the reaction of…
PMID 16187153doi:10.1007/s00284-005-0065-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (55 values) from this paper: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 25 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 22.9 | 0.0092 | 2.5 × 10³ calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 5.2 | 0.135 | 39 calc. | pH 5.7, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 24 | — | — | pH 8.2, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 29 | 0.266 | 110 calc. | pH 9.3, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinolinein H2O | — | — | 173 | pH 8.2, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | — | — | 187 | 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 19 | 0.058 | 330 calc. | pH 11, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 113.7 | 1.233 | 92.21 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 0.44 | 0.045 | 9.8 calc. | pH 5.1, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | 8.8 | 0.48 | 18 calc. | pH 9.1, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygenin H2O | — | — | 18.3 | pH 9.1, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S | Arthrobacter nitroguajacolicus | dioxygen | — | — | 19.6 | 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/E224A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 28.2 | 0.0154 | 1.83 × 10³ calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/E224A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/E224A | Arthrobacter nitroguajacolicus | dioxygen | 79.1 | 0.711 | 111 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/E224A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/F219Y | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 22.8 | 0.0095 | 2.4 × 10³ calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/F219Y, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/F219Y | Arthrobacter nitroguajacolicus | dioxygen | 110.9 | 1.27 | 87.3 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/F219Y, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H102Q | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 10 | 0.0257 | 390 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H102Q, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H102Q | Arthrobacter nitroguajacolicus | dioxygen | 14.8 | 0.325 | 45.5 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H102Q, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H251A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.01 | 0.0055 | 1.8 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H251A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/H251A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.53 | 0.298 | 1.8 calc. | pH 11, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/H251A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S101A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 2.3 | 0.1907 | 12 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S101A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S101A | Arthrobacter nitroguajacolicus | dioxygen | 38.8 | 1.382 | 28.1 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S101A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S220N | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 12.2 | 0.0154 | 792 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S220N, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/S220N | Arthrobacter nitroguajacolicus | dioxygen | 18.3 | 0.357 | 51.3 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/S220N, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196A | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 1.3 | 0.0995 | 13 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196A, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196A | Arthrobacter nitroguajacolicus | dioxygen | 6.4 | 1.646 | 3.9 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196A, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196K | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 0.7 | 0.0399 | 18 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196K, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196K | Arthrobacter nitroguajacolicus | dioxygen | 3.4 | 1.6 | 2.1 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196K, dioxygen |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196R | Arthrobacter nitroguajacolicus | 1H-3-hydroxy-4-oxoquinoline | 1 | 0.0836 | 12 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196R, 1H-3-hydroxy-4-oxoquinoline |
| 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenasevariant C69S/Y196R | Arthrobacter nitroguajacolicus | dioxygen | 3.4 | 1.37 | 2.5 calc. | pH 8, 30 °C | Open record: 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase variant C69S/Y196R, dioxygen |