CatLogenzyme kinetics catalog

EC 1.13.12.7: firefly luciferase

OxidoreductasesOpen in Browse

CatLog read 22 records for EC 1.13.12.7 from 1 paper, for 1 organism. Names in these papers: luciferase (Luc).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 109 records for EC 1.13.12.7 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.13.12.7 in Browse.

Papers

PaperOrganismsRecords
Mutagenesis evidence that the partial reactions of firefly bioluminescence are catalyzed by different conformations of the luciferase C-terminal domainPhotinus pyralis22

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
luciferase (Luc)Photinus pyralisD-firefly luciferin0.1670.01511 calc.pH 7.8PMID 15683224Open
luciferase (Luc)Photinus pyralisD-luciferyl-O-adenosine monophosphate0.230.004748.9pH 7.8PMID 15683224Open
luciferase (Luc)Photinus pyralisdehydroluciferin12——pH 7.8PMID 15683224Open
luciferase (Luc)Photinus pyralisMg-ATP—0.16—pH 7.8PMID 15683224Open
luciferase (Luc)variant G446IPhotinus pyralisD-firefly luciferin0.020.0063.3 calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant G446IPhotinus pyralisD-luciferyl-O-adenosine monophosphate0.0210.00159.5pH 7.8PMID 15683224Open
luciferase (Luc)variant G446IPhotinus pyralisMg-ATP—0.073—pH 7.8PMID 15683224Open
luciferase (Luc)variant K443APhotinus pyralisD-firefly luciferin6.29 × 10⁻⁵0.00230.027 calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant K443APhotinus pyralisD-luciferyl-O-adenosine monophosphate1.01 × 10⁻⁴3.8 × 10⁻⁴0.27pH 7.8PMID 15683224Open
luciferase (Luc)variant K443APhotinus pyralisMg-ATP—0.0486—pH 7.8PMID 15683224Open
luciferase (Luc)variant K443A/K529APhotinus pyralisD-firefly luciferin1.57 × 10⁻⁷0.0672.3 × 10⁻⁶ calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant K443A/K529APhotinus pyralisD-luciferyl-O-adenosine monophosphate3.11 × 10⁻⁵5.5 × 10⁻⁴0.057pH 7.8PMID 15683224Open
luciferase (Luc)variant K443A/K529APhotinus pyralisMg-ATP—0.56—pH 7.8PMID 15683224Open
luciferase (Luc)variant K445QPhotinus pyralisD-firefly luciferin0.240.008927 calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant K445QPhotinus pyralisD-luciferyl-O-adenosine monophosphate0.320.003982pH 7.8PMID 15683224Open
luciferase (Luc)variant K445QPhotinus pyralisMg-ATP—0.069—pH 7.8PMID 15683224Open
luciferase (Luc)variant K529APhotinus pyralisD-firefly luciferin2.5 × 10⁻⁴0.230.0011 calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant K529APhotinus pyralisD-luciferyl-O-adenosine monophosphate0.1750.012613.9pH 7.8PMID 15683224Open
luciferase (Luc)variant K529APhotinus pyralisMg-ATP—1.2—pH 7.8PMID 15683224Open
luciferase (Luc)variant Q448APhotinus pyralisD-firefly luciferin0.08750.00615 calc.pH 7.8PMID 15683224Open
luciferase (Luc)variant Q448APhotinus pyralisD-luciferyl-O-adenosine monophosphate0.0950.002243.2pH 7.8PMID 15683224Open
luciferase (Luc)variant Q448APhotinus pyralisMg-ATP—0.341—pH 7.8PMID 15683224Open